RAT SERPH (Center) antibody

Antigen: RAT SERPH

Binding Site: Center

Clonality: Monoclonal

Host: Mouse

Reactivity: Human, Rat (Rattus)

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN658998

Additional Information

Gene ID: 12406

UniProt: P19324

Immunogen: This RAT SERPH antibody is generated from mouse immunized with a KLH conjugated synthetic peptide selected from the Center region of human RAT SERPH.

Isotype: IgG1

Description: Other names: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: This RAT Serpinh1 antibody is generated from mouse immunized with a KLH conjugated synthetic peptide selected from the Central region of RAT Serpinh1.

Molecular Weight: 46590 DA

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:50-200 Immunohistochemistry ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Restrictions: For Research Use only

Publications

Product: Ishida, Kubota, Yamamoto et al.: "Type I collagen in Hsp47-null cells is aggregated in endoplasmic reticulum and deficient in N-propeptide processing and fibrillogenesis." in: Molecular biology of the cell, Vol. 17, Issue 5, pp. 2346-55, 2006 (PubMed).

Wang, Li: "Adenovirus-mediated RNA interference against collagen-specific molecular chaperone 47-KDa heat shock protein suppresses scar formation on mouse wounds." in: Cell biology international, Vol. 32, Issue 5, pp. 484-93, 2008 (PubMed).

Zhou, Ma, Kong et al.: "Fog2 is critical for cardiac function and maintenance of coronary vasculature in the adult mouse heart." in: The Journal of clinical investigation, Vol. 119, Issue 6, pp. 1462-76, 2009 (PubMed).

Sansom, Griffiths, Faedo et al.: "The level of the transcription factor Pax6 is essential for controlling the balance between neural stem cell self-renewal and neurogenesis." in: PLoS genetics, Vol. 5, Issue 6, pp. e1000511, 2009 (PubMed).

Kakugawa, Mukae, Hishikawa et al.: "Localization of HSP47 mRNA in murine bleomycin-induced pulmonary fibrosis." in: Virchows Archiv : an international journal of pathology, Vol. 456, Issue 3, pp. 309-15, 2010 (PubMed).