TNF Receptor-Associated Factor 2 (TRAF2) antibody

Antigen: TNF Receptor-Associated Factor 2 (TRAF2)

Synonyms: TRAP, TRAP3, MGC:45012, LAP1, CAP-1, CRAF1, CD40bp, RNF85, MGC:3310, AI325259, amn, T-BAM, AI528849, AI851288, 2310003F17Rik, C630032O20Rik, MGC83471, TANK, TRAF2, LOC716907, LOC100218184, Traf2

Clonality: Monoclonal

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN659110

Additional Information

Alternative name: TRAF2

Gene ID: 7186

UniProt: Q12933, NP_066961.2

Immunogen: This TRAF2 monoclonal antibody is generated from mouse immunized with TRAF2 recombinant protein.

Isotype: IgG1 kappa

Description: Other names: TNF receptor-associated factor 2,TRAF2,TRAP3

Characteristics: Mouse Monoclonal Antibody (Mab)

Specificity: This TRAF2 monoclonal antibody is generated from mouse immunized with TRAF2 recombinant protein.

Molecular Weight: 55859 DA

Comments:

Background: The protein encoded by this gene is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins associate with, and mediate the signal transduction from members of the TNF receptor superfamily. This protein directly interacts with TNF receptors, and forms a heterodimeric complex with TRAF1. This protein is required for TNF-alpha-mediated activation of MAPK8/JNK and NF-kappaB. The protein complex formed by this protein and TRAF1 interacts with the inhibitor-of-apoptosis proteins (IAPs), and functions as a mediator of the anti-apoptotic signals from TNF receptors. The interaction of this protein with TRADD, a TNF receptor associated apoptotic signal transducer, ensures the recruitment of IAPs for the direct inhibition of caspase activation. BIRC2/c-IAP1, an apoptosis inhibitor possessing ubiquitin ligase activity, can unbiquitinate and induce the degradation of this protein, and thus potentiate TNF-induced apoptosis. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of only one transcript has been determined.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1000 Western blotting~~ 1:50~1000

Concentration: 0.25 mg/ml

Buffer: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Ubiquitin-related antibodies, Apoptosis/Necrosis, Signaling, Phospho-specific antibodies, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Publications

Product: Alvarez, Harikumar, Hait et al.: "Sphingosine-1-phosphate is a missing cofactor for the E3 ubiquitin ligase TRAF2." in: Nature, Vol. 465, Issue 7301, pp. 1084-8, 2010 (PubMed).