DPF2 antibody

Details for Product No. ABIN659113
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Antigen
Synonyms im:7146797, zgc:101789, Requiem, req, req-A, ubi-d4, xReq, REQ, UBID4, 2210010M07Rik, Req
Reactivity
Human, Hamster
(70), (34), (17), (15), (15), (4), (4), (3), (2)
Host
Mouse
(50), (20)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(58), (32), (13), (11), (9), (9), (7), (2), (2), (2), (2), (1)
Pubmed 4 references available
Quantity 0.1 mL
Options
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Catalog No. ABIN659113
291.50 $
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Immunogen This DPF2 monoclonal antibody is generated from mouse immunized with DPF2 recombinant protein.
Clone 226CT14-1-1-2
Isotype IgG3, kappa
Specificity This DPF2 monoclonal antibody is generated from mouse immunized with DPF2 recombinant protein.
Purification Ascites
Background The protein encoded by this gene is a member of the d4 domain family, characterized by a zinc finger-like structural motif. This protein functions as a transcription factor which is necessary for the apoptotic response following deprivation of survival factors. It likely serves a regulatory role in rapid hematopoietic cell growth and turnover. This gene is considered a candidate gene for multiple endocrine neoplasia type I, an inherited cancer syndrome involving multiple parathyroid, enteropancreatic, and pituitary tumors.
Synonyms: 226CT14.1.1.2
Molecular Weight 44155 DA
Gene ID 5977
NCBI Accession NP_006259
UniProt Q92785
Research Area Apoptosis/Necrosis, Cell Structure, Signaling, Protein Modifications, Phospho-specific antibodies, Transcription Factors
Application Notes WB = 1:1000-32000
Restrictions For Research Use only
Format Liquid
Buffer Crude ascites with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-DPF2 antibody DPF2 antibody western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the DPF2 antibody detected the DPF2 protein (arrow).
anti-DPF2 antibody (2) DPF2 antibody western blot analysis in CHO cell line lysates (35ug/lane).This demonstrates the DPF2 antibody detected the DPF2 protein (arrow).
Background publications Beausoleil, Jedrychowski, Schwartz et al.: "Large-scale characterization of HeLa cell nuclear phosphoproteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 33, pp. 12130-5, 2004 (PubMed).

Olsen, Blagoev, Gnad et al.: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).

Matsuyama, Takada, Yokoyama et al.: "Double PHD fingers protein DPF2 recognizes acetylated histones and suppresses the function of estrogen-related receptor alpha through histone deacetylase 1." in: The Journal of biological chemistry, Vol. 285, Issue 24, pp. 18166-76, 2010 (PubMed).

Tando, Ishizaka, Watanabe et al.: "Requiem protein links RelB/p52 and the Brm-type SWI/SNF complex in a noncanonical NF-kappaB pathway." in: The Journal of biological chemistry, Vol. 285, Issue 29, pp. 21951-60, 2010 (PubMed).

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