SPINT1 antibody (AA 451-529) (HRP)

Catalog No. ABIN669870

The HRP-conjugated Rabbit Polyclonal anti-SPINT1 antibody (ABIN669870) specifically detects SPINT1 in WB, ELISA, IHC (p) and IHC (fro). The antibody is reactive with Mouse samples.

Quick Overview for SPINT1 antibody (AA 451-529) (HRP) (ABIN669870)

Target: SPINT1 (serine Peptidase Inhibitor, Kunitz Type 1 (SPINT1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPINT1 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-SPINT1 Antibody (HRP)

Binding Specificity: AA 451-529

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human HAI-1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for SPINT1

Target: SPINT1 (serine Peptidase Inhibitor, Kunitz Type 1 (SPINT1))

Alternative Name: HAI-1

Background:

Synonyms: Hepatocyte Growth Factor Activator Inhibitor 1, Spint1, Hai1, HGFA Inhibitor 1, HAI-1, SPIT1_HUMAN.

Background: HGFA Inhibitor 1 is an endogenous inhibitor of the kunitz-type serine proteinase HGF-activator (HGFA). HGF was first described as a hepatocyte-specific mitogen and survival factor, and has since been shown to exert a variety of actions on many cell types by binding to its MET receptor. HGF is activated by cleavage of the single-chain form to form a two-chain version by HGFA. HGFA Inhibitor 1 also inhibits trypsin, and MTSP-1 (Matriptase), a serine proteinase that also activates HGF. HGFB Inhibitor 1 is a splice variant of HGFA Inhibitor 1, and contains a 16 amino acid insert after the first kringle domain, relative to the HGFA Inhibitor 1 sequence.

Gene ID: 6692