ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 1B (ABCB1B) (AA 21-100) antibody

Catalog No. ABIN670161

Product Details

Target: ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 1B (ABCB1B)

Binding Specificity: AA 21-100

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from mouse MDR1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References

Wei, Liang, Junsong, Rui, Shuai, Guanglin, Shicai, Zexing, Jin, Xiangming, Shufeng: "The impact of insulin on chemotherapeutic sensitivity to 5-fluorouracil in gastric cancer cell lines SGC7901, MKN45 and MKN28." in: Journal of experimental & clinical cancer research : CR, Vol. 34, pp. 64, (2016) (PubMed).

Sun, Jiao, Chen, Liu, Zhao: "MicroRNA-186 induces sensitivity of ovarian cancer cells to paclitaxel and cisplatin by targeting ABCB1." in: Journal of ovarian research, Vol. 8, pp. 80, (2016) (PubMed).

Peng, Feng, Yuan, Benhabbour, Mumper: "Development of a novel orthotopic non-small cell lung cancer model and therapeutic benefit of 2'-(2-bromohexadecanoyl)-docetaxel conjugate nanoparticles." in: Nanomedicine : nanotechnology, biology, and medicine, Vol. 10, Issue 7, pp. 1497-506, (2014) (PubMed).

Hu, Peng, Li: "The expression and significance of P-glycoprotein, lung resistance protein and multidrug resistance-associated protein in gastric cancer." in: Journal of experimental & clinical cancer research : CR, Vol. 28, pp. 144, (2009) (PubMed).

Target Details

Target: ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 1B (ABCB1B)

Alternative Name: MDR1 (ABCB1B Products)

Background:

Synonyms: Pgp, P-gp, Evi32, Mdr1a, Multidrug resistance protein 1A, ATP-binding cassette sub-family B member 1A, Abcb1a

Background: Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.

Gene ID: 18671

UniProt: P21447