CKIP-1 antibody

Antigen: CKIP-1

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus), Mouse (Murine)

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Flow Cytometry (FACS), Immunofluorescence (IF)

Catalog no.: ABIN675039

Additional Information

Immunogen: Polyclonal antibodies are produced by immunizing animals with synthetic peptide derived from human CKIP-1 C-terminus

Format: Lyophilized

Reconstitution: Reconstitute with 100ul sterille distilled water. After reconstitution, stock concentration=1ug/1ul, preservatives included (BSA=10ug/ul, Sodium azide=0.1%)

Isotype: IgG  (Matching secondary antibodies)

Description: Synonym: PLEKHO1, C-Jun-binding protein, Casein kinase 2-interacting protein 1, CK2 interacting protein 1, CK2 interacting protein 1 HQ0024c protein, CK2-interacting protein 1, CKIP 1, CKIP1, JBP, OC120, Osteoclast maturation associated gene 120 protein, Pleckstrin homology domain containing family O member 1.

Molecular Weight: 45kDa

Comments:

Plays a role in the regulation of the actin cytoskeleton through its interactions with actin capping protein (CP). May function to target CK2 to the plasma membrane thereby serving as an adapter to facilitate the phosphorylation of CP by protein kinase 2(CK2). Appears to target ATM to the plasma membrane. Appears to also inhibit tumor cell growth by inhibiting AKT-mediated cell-survival. Also implicated in PI3K-regulated muscle differentiation

the regulation of AP-1 activity (plasma membrane bound AP-1 regulator that translocates to the nucleus) and the promotion of apoptosis induced by tumor necrosis factor TNF. When bound to PKB

it inhibits it probably by decreasing PKB level of phosphorylation. [SUBUNIT] Heterodimer or homodimer. [SUBCELLULAR LOCATION] Cell membrane. Nucleus. Cytoplasm. [TISSUE SPECIFICITY] Abundantly expressed in skeletal muscle and heart

moderately in kidney

liver

brain and placenta and sparingly in the pancreas and lung. Easily detectable in cancer cell lines such as MOLT-4

HEK293 and Jurkat cells.

Application Details

Application Notes: WB (1:100-500), ELISA (1:500-1000), IP (1:20-100), IHC-P (1:100-500), IHC-F (1:100-500), Flow-Cyt (1:20-100), IF (1:50-200)

Concentration: 1mg/1ml

Purification: affinity purified by Protein A

Buffer: 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

Storage: Ship at room temperature. Store at -20°C

Restrictions: For Research Use only