E Tag antibody
| Antigen |
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| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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 Alternatives Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Flow Cytometry (FACS), Immunofluorescence (IF)
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| Catalog no. |
ABIN677753 |
| Quantity |
100 µg (1mg/1ml) |
| Price |
255.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 to 7 Business Days |
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Immunogen
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Polyclonal antibodies are produced by immunizing animals with E Tag peptide
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Format
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Lyophilized
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Reconstitution
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Reconstitute with 100ul sterille distilled water. After reconstitution, stock concentration=1ug/1ul, preservatives included (BSA=10ug/ul, Sodium azide=0.1%)
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Isotype
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IgG (Matching secondary antibodies)
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Comments
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E Tag is a commonly used epitope tag engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized. Because of the small size of the epitope it is unlikely to alter the activity of the cloned sequence.
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Application Notes
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WB (1:100-500), ELISA (1:500-1000), IP (1:20-100), IHC-P (1:100-500), IHC-F (1:100-500), Flow-Cyt (1:20-100), IF (1:50-200)
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Concentration
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1mg/1ml
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Purification
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affinity purified by Protein A
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Buffer
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0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
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Storage
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Ship at room temperature. Store at -20°C
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Restrictions
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For Research Use only
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Alternatives for antigen "E Tag", type "Antibodies"
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Hosts
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Rabbit (34), Chicken (5), Goat (5), Mouse (2)
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Reactivities
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Fusion Protein (Tag) (13), Tag (6), Human (4), Mouse (Murine) (4), Rat (Rattus) (4)
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Applications
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ELISA (29), Western Blotting (WB) (26), Immunofluorescence (IF) (18), Flow Cytometry (FACS) (17), Immunocytochemistry (ICC) (7), Dot Blot (Dot) (3), Immunoprecipitation (IP) (2), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (1), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (1)
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Conjugates
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HRP (8), Biotin (2), FITC (2), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Gold (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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