Histone H1 antibody (Biotin)

Antigen: Histone H1

Synonyms: H1c, H1.2, H1f2, His1a, H1var1, MGC107646, 0610008C09Rik, LOC692942, HISTONE H1, HON4

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus), Rabbit, Horse (Equine), Pig (Porcine), Cow (Bovine), Dog (Canine)

Conjugate: Biotin

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Flow Cytometry (FACS), Immunofluorescence (IF)

Catalog no.: ABIN686199

Additional Information

Immunogen: Polyclonal antibodies are produced by immunizing animals with synthetic peptide derived from human Histone H1 C-terminus

Format: Lyophilized

Reconstitution: Reconstitute with 100ul sterille distilled water. After reconstitution, stock concentration=1ug/1ul, preservatives included (BSA=10ug/ul, Sodium azide=0.1%)

Isotype: IgG

Description: Synonym: H1oo, Oocyte specific linker histone H1, dJ221C16.2, dJ221C16.5, DmeH1, H1 0 histone, H1, H1 histone family member 0, H1 histone family member 1, H1 histone family member 3, H1 histone family member 5, H1 histone family member N testis specific, H1 histone family member O oocyte specific, H1 histone family member X, H1(0), H1.1, H1.2, H1.3, H1.4, H1.5, H10, H1A, H1f0, H1F0 protein, H1F1, H1F2, H1F3, H1F4, H1F5, H1FNT, H1FOO, H1FOO protein, H1FT, H1FV, H1FX, H1t, H1T2, H1X, HANP1, His1, HisC, HIST1, HIST1H1A, HIST1H1B, HIST1H1C, HIST1H1D, HIST1H1E, HIST1H1T, Histone 1 H1a, Histone 1 H1b, Histone 1 H1c, Histone 1 H1d, Histone 1 H1e, Histone 1 H1t, Histone, Histone H1, Histone H1.0, Histone H1.1, Histone H1.2, Histone H1.3, Histone H1.4, Histone H1.5, Histone H1x, MGC116819, MGC126630, MGC126632, MGC126642, MGC138176, MGC138345, MGC15959, MGC3992, MGC50807, MGC5241, MGC8350, Oocyte specific histone H1, osH1, Testicular H1 histone.

Molecular Weight: 22kDa

Comments:

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A

H2B

H3

and H4) form an octamer

around which approximately 146 bp of DNA is wrapped in repeating units

called nucleosomes. The linker histone

H1

interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.

Application Details

Application Notes: WB (1:100-500), ELISA (1:500-1000), IP (1:20-100), IHC-P (1:100-500), IHC-F (1:100-500), Flow-Cyt (1:20-100), IF (1:50-200)

Concentration: 1mg/1ml

Purification: Antibodies are purified by protein A and peptide affinity chromatography.

Buffer: 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

Storage: Ship at room temperature. Store at -20°C

Research Area: Chromatin and Nuclear Signaling, DNA/RNA, Transcription Factors

Restrictions: For Research Use only