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Histone 3 antibody (H3K9ac) (Cy3)

The Rabbit Polyclonal anti-Histone 3 antibody is suitable to detect Histone 3 in samples from Human, Mouse and Rat. It has been validated for WB, FACS, IF (cc) and IF (p).
Catalog No. ABIN703949
$470.46
Plus shipping costs $50.00
100 μL
Shipping to: United States
Delivery in 16 to 20 Business Days

Quick Overview for Histone 3 antibody (H3K9ac) (Cy3) (ABIN703949)

Target

See all Histone 3 (H3) Antibodies
Histone 3 (H3) (Histone H3 (H3))

Reactivity

  • 1724
  • 1151
  • 1017
  • 48
  • 40
  • 37
  • 36
  • 32
  • 25
  • 23
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  • 9
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Human, Mouse, Rat

Host

  • 1494
  • 262
  • 8
  • 5
  • 1
Rabbit

Clonality

  • 1116
  • 653
  • 1
Polyclonal

Conjugate

  • 947
  • 103
  • 67
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This Histone 3 antibody is conjugated to Cy3

Application

  • 1380
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Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))
  • Binding Specificity

    • 69
    • 52
    • 52
    • 51
    • 48
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    • 40
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    H3K9ac

    Specificity

    This acetylation site is homologous across the species listed.

    Cross-Reactivity

    Human, Mouse, Rat

    Predicted Reactivity

    Cow,Pig,Rabbit,Drosophila

    Purification

    Purified by Protein A.

    Immunogen

    KLH conjugated synthetic acetylpeptide derived from human Histone H3 around acetylation site of Lys9

    Isotype

    IgG
  • Application Notes

    FCM 1:20-100
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 μg/μL

    Buffer

    Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

    Preservative

    ProClin

    Precaution of Use

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

    Storage

    -20 °C

    Storage Comment

    Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

    Expiry Date

    12 months
  • Target

    Histone 3 (H3) (Histone H3 (H3))

    Alternative Name

    Histone H3

    Background

    Synonyms: H3F3, H3.3A, Histone H3.3, H3F3A, PP781

    Background: Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

    Gene ID

    3020

    UniProt

    P84243
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