alpha Fetoprotein antibody (HRP)

Catalog No. ABIN722925

The HRP-conjugated Mouse Monoclonal anti-alpha Fetoprotein antibody (Clone A4) (ABIN722925) specifically detects alpha Fetoprotein in WB, ELISA and IHC (p). The antibody is reactive with Human samples.

Quick Overview for alpha Fetoprotein antibody (HRP) (ABIN722925)

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This alpha Fetoprotein antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: A4

Product Details anti-alpha Fetoprotein Antibody (HRP)

Cross-Reactivity: Human

Purification: Purified by Protein G.

Immunogen: Full-length AFP protein

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for alpha Fetoprotein

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Alternative Name: AFP

Background:

Synonyms: AFPD, FETA, HPAFP, Alpha-fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin, AFP

Background: Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2 % ) of the human AFP shows estrogen-binding properties.

Gene ID: 174

UniProt: P02771

Pathways: C21-Steroid Hormone Metabolic Process