PACAP-27/38 (AA 132-169) antibody (HRP)

Catalog No. ABIN726074

The Rabbit Polyclonal anti-PACAP-27/38 antibody is suitable to detect PACAP-27/38 in samples from Human and Rat. It has been validated for WB, ELISA, IHC (fro) and IHC (p).

Quick Overview for PACAP-27/38 (AA 132-169) antibody (HRP) (ABIN726074)

Target: PACAP-27/38

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details

Binding Specificity: AA 132-169

Cross-Reactivity: Human, Rat

Predicted Reactivity: Mouse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human PACAP-27/38

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: PACAP-27/38

Background:

Synonyms: PACAP, Pituitary adenylate cyclase-activating polypeptide, ADCYAP1, PACAP-27, PACAP27, PACAP-38, PACAP38.

Background: Binding to its receptor activates G proteins and stimulates adenylate cyclase in pituitary cells. Promotes neuron projection development through the RAPGEF2/Rap1/B-Raf/ERK pathway.

Gene ID: 116

UniProt: P18509