Runx1 + Runx2 (AA 202-300) antibody

Catalog No. ABIN738156

Cited in 6+ publications. The Rabbit Polyclonal anti-Runx1 + Runx2 antibody (ABIN738156) specifically detects Runx1 + Runx2 in WB, FACS, IF (cc), IF (p), ELISA, IHC (fro) and IHC (p). The antibody is reactive with Guinea Pig, Human, Mouse, Rabbit and Rat samples.

Quick Overview for Runx1 + Runx2 (AA 202-300) antibody (ABIN738156)

Target: Runx1 + Runx2

Reactivity: Guinea Pig, Human, Mouse, Rabbit, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details

Binding Specificity: AA 202-300

Cross-Reactivity: Guinea Pig, Human, Mouse, Rabbit, Rat

Predicted Reactivity: Dog,Cow,Sheep,Horse,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human RUNX2

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References

Sun, Kiraly, Cox, Mauerhan, Hanley: "The role of inhibition by phosphocitrate and its analogue in chondrocyte differentiation and subchondral bone advance in Hartley guinea pigs." in: Experimental and therapeutic medicine, Vol. 15, Issue 4, pp. 3320-3328, (2018) (PubMed).

Li, Deng, Wei, Jayasuriya, Zhou, Chen, Zhang, Wei, Wei: "Blockade of hypoxia-induced CXCR4 with AMD3100 inhibits production of OA-associated catabolic mediators IL-1β and MMP-13." in: Molecular medicine reports, Vol. 14, Issue 2, pp. 1475-82, (2016) (PubMed).

Hu, Wang, Zhou, Shang: "High expression of periostin is dramatically associated with metastatic potential and poor prognosis of patients with osteosarcoma." in: World journal of surgical oncology, Vol. 12, pp. 287, (2014) (PubMed).

Zhang, Men, Fu, Shan, Ye, Wu, Tao, Liu, Jiang: "Contribution of SATB2 to the stronger osteogenic potential of bone marrow stromal cells from craniofacial bones." in: Cell and tissue research, Vol. 350, Issue 3, pp. 425-37, (2013) (PubMed).

Gao, Zhao, Song, Yang: "Expression pattern of embryonic stem cell markers in DFAT cells and ADSCs." in: Molecular biology reports, Vol. 39, Issue 5, pp. 5791-804, (2012) (PubMed).

Target Details

Target: Runx1 + Runx2

Alternative Name: RUNX1+RUNX2

Background:

Synonyms: CCD, AML3, CCD1, CLCD, OSF2, CBFA1, OSF-2, PEA2aA, PEBP2aA, CBF-alpha-1, Runt-related transcription factor 2, Acute myeloid leukemia 3 protein, Core-binding factor subunit alpha-1, Oncogene AML-3, Osteoblast-specific transcription factor 2, Polyomavirus enhancer-binding protein 2 alpha A subunit, PEA2-alpha A, PEBP2-alpha A, SL3-3 enhancer factor 1 alpha A subunit, SL3/AKV core-binding factor alpha A subunit, RUNX2, PEBP2A

Background: Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters. In osteoblasts, supports transcription activation: synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE) (By similarity). Inhibits KAT6B-dependent transcriptional activation.

Gene ID: 860

UniProt: Q13950