AGEs antibody

Antigen: AGEs

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus), Mouse (Murine)

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunofluorescence (IF)

Catalog no.: ABIN738516

Additional Information

Immunogen: Polyclonal antibodies are produced by immunizing animals with AGEs

Format: Lyophilized

Reconstitution: Reconstitute with 100ul sterille distilled water. After reconstitution, stock concentration=1ug/1ul, preservatives included (BSA=10ug/ul, Sodium azide=0.1%)

Isotype: IgG  (Matching secondary antibodies)

Description: Synonym: advanced glycosylation end products, AGE.

Comments:

Advanced Glycation End products (AGEs) are the result of a chain of chemical reactions after an initial glycation reaction. The intermediate products are known

variously

as Amadori

Schiff base and Maillard products

named after the researchers who first described them. (The literature is inconsistent in applying these terms. For example

Maillard reaction products are sometimes considered intermediates and sometimes end products.) Side products generated in intermediate steps may be oxidizing agents (such as hydrogen peroxide)

or not (such as beta amyloid proteins).[1] "Glycosylation" is sometimes used for "glycation" in the literature

usually as 'non-enzymatic glycosylation. The AGE modified BSA was produced by reacting BSA with glycolaldehyde under sterile conditions followed by extensive dialysis and purification steps.

Application Details

Application Notes: ELISA (1:500-1000), IHC-P (1:100-500), IHC-F (1:100-500), IF (1:50-200)

Concentration: 1mg/1ml

Purification: affinity purified by Protein A

Buffer: 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

Storage: Ship at room temperature. Store at -20°C

Research Area: Immunology, Metabolism, Diabetes

Restrictions: For Research Use only