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+1 404 474 4654 |
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+1 888 205 9894 (TF) |
Apelin (APLN) antibody
| Antigen | Apelin (APLN) |
| Synonyms | Apel, 6030430G11Rik, XNPEP2, APLN, Xapelin, xnpep2, preproapelin |
| Clonality | Polyclonal |
| Host |
 Alternatives Rabbit |
| Reactivity |
Alternatives Human, Mouse (Murine), Rat (Rattus) |
| Application |
Alternatives Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Flow Cytometry (FACS), Immunofluorescence (IF)
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| Catalog no. | ABIN740085 |
| Quantity | 100 µg (1mg/1ml) |
| Price | 255.00 $ Plus shipping costs $35.00 |
| Shipping to |
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| Availability | Ships within 5 to 7 Business Days |
Additional Information
| Alternative name | Apelin |
| Immunogen | Polyclonal antibodies are produced by immunizing animals with synthetic peptide derived from human Apelin C-terminus |
| Format | Lyophilized |
| Reconstitution | Reconstitute with 100ul sterille distilled water. After reconstitution, stock concentration=1ug/1ul, preservatives included (BSA=10ug/ul, Sodium azide=0.1%) |
| Isotype | IgG (Matching secondary antibodies) |
| Description | Synonym: APLN, Apel, AGTRL1 ligand, APEL, APJ endogenous ligand, XNPEP2. |
| Molecular Weight | 10kDa |
| Comments |
Apelin is a neuropeptide expressed in the supraoptic and paraventricular nuclei and is an endogenous ligand for APJ a G protein-coupled orphan receptor which is an alternative coreceptor with CD4 for HIV-1. Apelin and APJ are ubiquitously expressed in peripheral tissues with highest levels reported for heart and lungs as well several regions within the central nervous system. The actions of apelin remain largely unknown: Apelin inhibits HIV-1 entry in cells coexpressing CD4 and APJ the oral intake of Apelin in colostrum and breast milk could have a role in the modulation of the immune responses in neonates more recent studies have also indicated a role for Apelin in the central control of body fluid homeostasis by influencing AVP release and drinking behavior. In the cardiovascular system several actions of Apelin have been described including endothelium-dependent vasodilatation vasoconstriction through direct action on smooth muscle and positive inotropism. |
Application Details
| Application Notes | WB (1:100-500), ELISA (1:500-1000), IP (1:20-100), IHC-P (1:100-500), IHC-F (1:100-500), Flow-Cyt (1:20-100), IF (1:50-200) |
| Concentration | 1mg/1ml |
| Purification | affinity purified by Protein A |
| Buffer | 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide |
| Storage | Ship at room temperature. Store at -20°C |
| Research Area | Cardiovascular, Contractility |
| Restrictions | For Research Use only |
Alternatives
| Hosts | Rabbit (26), Mouse (5) |
| Reactivities | Human (30), Mouse (Murine) (21), Rat (Rattus) (21), Cow (Bovine) (4), Dog (Canine) (2), Pig (Porcine) (2), Chicken (1), Horse (Equine) (1), Rabbit (1) |
| Applications | Flow Cytometry (FACS) (17), Immunofluorescence (IF) (17), Western Blotting (WB) (11), Enzyme Immunoassay (EIA) (5), ELISA (4), Dot Blot (Dot) (2), ELISA (Detection) (2), Immunohistochemistry (IHC) (2), Immunoassay (IA) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (1), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (1), Immunoprecipitation (IP) (1) |
| Conjugates | Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1) |
