PAK1 , PAK2 , PAK3 T423 , T402 , T421 (pThr402), (pThr421), (pThr423) antibody

Catalog No. ABIN2178181

This Rabbit Polyclonal antibody specifically detects PAK1 , PAK2 , PAK3 T423 , T402 , T421 in ELISA, IF (cc), IF (p), IHC (fro), IHC (p) and WB. It exhibits reactivity toward Rat.

Quick Overview for PAK1 , PAK2 , PAK3 T423 , T402 , T421 (pThr402), (pThr421), (pThr423) antibody (ABIN2178181)

Target: PAK1 , PAK2 , PAK3 T423 , T402 , T421

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Application: ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Product Details

Binding Specificity: pThr402, pThr421, pThr423

Cross-Reactivity: Rat

Predicted Reactivity: Human,Mouse,Dog,Cow,Pig,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human PAK1 around the phosphorylation site of Thr423

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: PAK1 , PAK2 , PAK3 T423 , T402 , T421

Alternative Name: PAK1 + PAK2 + PAK3 T423 + T402 + T421

Background:

Synonyms: PAK1 + PAK2 + PAK3 phospho T423 + T402 + T421, PAK1 + PAK2 + PAK3 phospho Thr423 + Thr402 + Thr421, Pak2phospho T402, Pak1phospho T423, PAK1phospho T402, PAK2phospho T423, PAKalpha, serine/threonine-protein kinase PAK 1, p21 activated kinase 1, PAK alpha, Paka, PAKalpha, Protein kinase MUK2, STE20 homolog yeast, Alpha PAK, p21/Cdc42/Rac1 activated kinase 1, CB422, EC 2.7.11.1, Gamma PAK, hPAK65, Kinase, p21 CDKN1A-activated kinase 2a, p21 activated kinase 2, p21 protein Cdc42/Rac-activated kinase 2, p21 protein Cdc42 Rac activated kinase 2, p21-activated kinase, 65-KD, p21-activated protein kinase I, p21CDKN1A activated kinase 2, p58, p65PAK, PAK 2, PAK65, PAKgamma, S6 H4 kinase, Serine threonine protein kinase PAK 2, Serine/threonine-protein kinase PAK 3, p21-activated kinase 3, PAK-3, Beta-PAK, PAK1_HUMAN, PAK2_HUMAN.

Background: In mammals, there are several identified isoforms of p21 Activated Protein Kinases or PAKs: PAK1 and PAK3 are mostly brain specific, while PAK2 is expressed ubiquitously. Mutations of the gene coding for PAK3 are associated with X linked mental retardation and PAK3 is a key regulator of synapse formation and plasticity in the hippocampus. PAK3 is thought to play a key role in regulation of cell shape and motility as well as cell death. P21-activated kinase (PAK) is actually a family of serine/threonine protein kinases, members of which are activated by small molecular weight GTPases. The three most common isoforms are PAK 1, PAK 2, and PAK 3 (also known as alpha PAK, gamma PAK, and beta PAK, respectively). These kinases contain numerous regulatory elements that trigger diverse signaling processes such as those initiated by activated GTPases, interaction with Src homology 3 (SH3) domains, and caspase mediated proteolytic cleavage. Autophosphorylation of serine 141 (serine 144 for PAK 1 and serine 139 PAK 3), catalyzed by Cdc42, is required for activation of PAK.

Gene ID: 5058