PRKACG antibody (AA 251-351) (HRP)

Catalog No. ABIN751822

This Rabbit Polyclonal antibody specifically detects PRKACG in WB, ELISA, IHC (p) and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for PRKACG antibody (AA 251-351) (HRP) (ABIN751822)

Target: PRKACG (Protein Kinase, CAMP-Dependent, Catalytic, gamma (PRKACG))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRKACG antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-PRKACG Antibody (HRP)

Binding Specificity: AA 251-351

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Sheep,Pig,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human PKA gamma

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for PRKACG

Target: PRKACG (Protein Kinase, CAMP-Dependent, Catalytic, gamma (PRKACG))

Alternative Name: PKA gamma

Background:

Synonyms: KAPG, PKA C gamma, PRKACG, Protein kinase cAMP dependent catalytic gamma, Serine threonine protein kinase, KAPCG_HUMAN.

Background: PKA (or cAPK) is a cyclic AMP dependent protein kinase. When activated by the second messenger cAMP, PKA mediates diverse cellular mechanisms, including proliferation, ion transport, regulation of metabolism, plus gene transcription. PKA is comprised of two dimers of two subunits, R (regulatory) and C (catalytic). Two families of R subunit (RI and RII) and three C subunit isoforms (C alpha, C beta, and C gamma) have been identified each possessing distinct cAMP binding properties and resulting in different phosphorylation states. C subunit is activated through autophosphorylation and direct phosphorylation at Thr197 by PDK-1. Tissue specific expression of C gamma, indicates pressure on C gamma during evolution, acting to modulate it in a functionally specific way. Certain amino acid substitutions make C gamma a distinct member of the cAMP dependent subfamily of protein kinases, and suggest that C gamma may be distinct in its protein substrate specificity or its interaction with the different regulatory subunits.

Gene ID: 5568

Pathways: AMPK Signaling, Hedgehog Signaling, Thyroid Hormone Synthesis, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Lipid Metabolism