PKA beta (pSer338) antibody

Catalog No. ABIN752698

This Rabbit Polyclonal antibody specifically detects PKA beta in ELISA, WB, ICC, IF (cc), IF (p), IHC (fro) and IHC (p). It exhibits reactivity toward Mouse.It has been mentioned in 1 publication

Quick Overview for PKA beta (pSer338) antibody (ABIN752698)

Target: PKA beta

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details

Binding Specificity: pSer338

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Dog,Cow,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human PKA beta around the phosphorylation site of Ser338

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References

Wu, Zhu, Yang, Wang, Wang, Wang, Gao, Zhang, Jiang, Zhu, Shu: "N-Oleoylglycine-Induced Hyperphagia Is Associated with the Activation of Agouti-Related Protein (AgRP) Neuron by Cannabinoid Receptor Type 1 (CB1R)." in: Journal of agricultural and food chemistry, Vol. 65, Issue 5, pp. 1051-1057, (2017) (PubMed).

Target Details

Target: PKA beta

Background:

Synonyms: PKA C beta, PKACB, PRKACB, Protein kinase cAMP dependent catalytic beta, cAMP-dependent protein kinase catalytic subunit beta, KAPCB_HUMAN.

Background: cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is a member of the Ser/Thr protein kinase family and is a catalytic subunit of cAMP-dependent protein kinase. Several alternatively spliced transcript variants encoding distinct isoforms have been observed.

Gene ID: 5567