SLC29A4 antibody (AA 401-500) (HRP)

Catalog No. ABIN754957

This Rabbit Polyclonal antibody specifically detects SLC29A4 in WB, ELISA, IHC (p) and IHC (fro). It exhibits reactivity toward Human and Rat.

Quick Overview for SLC29A4 antibody (AA 401-500) (HRP) (ABIN754957)

Target: SLC29A4 (Solute Carrier Family 29 (Nucleoside Transporters), Member 4 (SLC29A4))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC29A4 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-SLC29A4 Antibody (HRP)

Binding Specificity: AA 401-500

Cross-Reactivity: Human, Rat

Predicted Reactivity: Mouse,Dog,Cow,Pig,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human SLC29A4

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for SLC29A4

Target: SLC29A4 (Solute Carrier Family 29 (Nucleoside Transporters), Member 4 (SLC29A4))

Alternative Name: SLC29A4

Background:

Synonyms: ENT4, PMAT, Equilibrative nucleoside transporter 4, hENT4, Plasma membrane monoamine transporter, Solute carrier family 29 member 4, SLC29A4, PSEC0113

Background: Functions as a polyspecific organic cation transporter, efficiently transporting many organic cations such as monoamine neurotransmitters 1-methyl-4-phenylpyridinium and biogenic amines including serotonin, dopamine, norepinephrine and epinephrine. May play a role in regulating central nervous system homeostasis of monoamine neurotransmitters. May be involved in luminal transport of organic cations in the kidney and seems to use luminal proton gradient to drive organic cation reabsorption. Does not seem to transport nucleoside and nucleoside analogs such as uridine, cytidine, thymidine, adenosine, inosine, guanosine, and azidothymidine. In (PubMed:16873718) adenosine is efficiently transported but in a fashion highly sensitive to extracellular pH , with maximal activity in the pH range 5.5 to 6.5. Glu-206 is essential for the cation selectivity and may function as the charge sensor for cationic substrates. Transport is chloride and sodium-independent but appears to be sensitive to changes in membrane potential. Weakly inhibited by the classical inhibitors of equilibrative nucleoside transport, dipyridamole, dilazep, and nitrobenzylthioinosine. May play a role in the regulation of extracellular adenosine concentrations in cardiac tissues, in particular during ischemia.

Gene ID: 222962

UniProt: Q7RTT9