MYL1 antibody (AA 101-170) (Cy7)

Catalog No. ABIN755713

This Rabbit Polyclonal antibody specifically detects MYL1 in WB, IF (cc) and IF (p). It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for MYL1 antibody (AA 101-170) (Cy7) (ABIN755713)

Target: MYL1 (Myosin, Light Chain 1, Alkali, Skeletal, Fast (MYL1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MYL1 antibody is conjugated to Cy7

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-MYL1 Antibody (Cy7)

Binding Specificity: AA 101-170

Specificity: This antibody may recognize myosin light chain 5, myosin light chain 7, or myosin regulatory light chain 10

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Sheep,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human MRLC2

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for MYL1

Target: MYL1 (Myosin, Light Chain 1, Alkali, Skeletal, Fast (MYL1))

Alternative Name: Fast skeletal Myosin

Background:

Synonyms: MRLC2, MYL11, HUMMLC2B, Myosin regulatory light chain 2, skeletal muscle isoform, Fast skeletal myosin light chain 2, MLC2B, MYLPF

Background: Myosin is a highly conserved, ubiquitously expressed protein that interacts with Actin to generate the force for cellular movements. Conventional Myosins are hexameric proteins consisting of two heavy chain subunits, a pair of non-phosphorylatable light chain subunits and a pair of phosphorylatable light chain subunits. Three general classes of Myosin have been cloned: smooth muscle Myosins, striated muscle Myosins and non-muscle Myosins . Contractile activity in smooth muscle is regulated by the calcium/calmodulin-dependent phosphorylation of Myosin light chain (MLC) by Myosin light chain kinase. Myosin heavy chains, which are encoded by the MYH gene family, contain Actin-activated ATPase activity which generates the motor function of Myosin. Myosin heavy chains were initially isolated from a human fetal skeletal muscle and are the major determinant in the speed of contraction of skeletal muscle. Various isoforms of myosin heavy chains are differentially expressed depending on the functional activity of the muscle.

Gene ID: 29895

UniProt: Q96A32