HIF3 alpha antibody

Antigen: HIF3 alpha

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine)

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Flow Cytometry (FACS), Immunofluorescence (IF)

Catalog no.: ABIN759506

Additional Information

Immunogen: Polyclonal antibodies are produced by immunizing animals with synthetic peptide derived from human HIF3 alpha

Format: Lyophilized

Reconstitution: Reconstitute with 100ul sterille distilled water. After reconstitution, stock concentration=1ug/1ul, preservatives included (BSA=10ug/ul, Sodium azide=0.1%)

Isotype: IgG  (Matching secondary antibodies)

Description: Synonym: HIF 3 alpha, HIF-3 Alpha, HIF 3A, HIF 3A4, HIF3 alpha, HIF3A, HIF 3A, HIF-3A, Hypoxia Inducible Factor 3 alpha, Hypoxia inducible factor 3 alpha subunit, Hypoxia inducible factor three alpha, Inhibitory PAS domain protein, IPAS, MOP7, PASD7.

Molecular Weight: 60-72kDa

Comments:

Hypoxia-inducible factor (HIF) is one of the most important factors in the cellular response to hypoxia

transcriptionally activating genes encoding proteins that mediate adaptive responses to reduced oxygen availability. HIF is a heterodimer consisting of one of three subunits

HIF1 alpha

HIF2 alpha

or HIF3 alpha. HIF target genes play critical roles in metabolism

angiogenesis

cell proliferation and cell survival. HIF3 alpha protein is one of several alpha/beta-subunit heterodimeric transcription factors that regulate many adaptive responses to low oxygen tension (hypoxia). The alpha 3 subunit lacks the transactivation domain found in factors containing either the alpha 1 or alpha 2 subunits. HIF3 alpha may be a marker for tumor growth and angiogenesis.

Application Details

Application Notes: WB (1:100-500), ELISA (1:500-1000), IP (1:20-100), IHC-P (1:100-500), IHC-F (1:100-500), Flow-Cyt (1:20-100), IF (1:50-200)

Concentration: 1mg/1ml

Purification: affinity purified by Protein A

Buffer: 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

Storage: Ship at room temperature. Store at -20°C

Research Area: Cancer, Transcription Factors

Restrictions: For Research Use only