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Antibodies
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anti-POU Class 5 Homeobox 1 (POU5F1) antibody
POU Class 5 Homeobox 1 (POU5F1) antibody
| Antigen |
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| Synonyms |
OCT3, OCT4, OTF3, OTF4, MGC22487, POU5F1, POU5F1L |
| Clonality |
Monoclonal |
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Host
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Reactivity
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Application
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| Catalog no. |
ABIN781220 |
| Quantity |
100ug (100µg/100µl) (Variants) |
| Price |
406.67 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 to 7 Business Days |
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Alternative name
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POU5F1
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Swiss-Prot
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NM_013633
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Immunogen
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Recombinant full length of Mouse POU5F1.
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Format
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Liquid
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Isotype
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IgG (Matching secondary antibodies)
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Description
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POU5F1(Oct-4) is a transcription factor highly expressed in undifferentiated embryonic stem cells and embryonic germ cells. A network of key factors that includes Oct-4, Nanog, and Sox2 is necessary for the maintenance of pluripotent potential, and downregulation of Oct-4 has been shown to trigger cell differentiation. Oct-4 is a useful germ cell tumor marker. Oct-4 exists as two splice variants, Oct-4A and Oct-4B.
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Specificity
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Nucleus
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Molecular Weight
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45kDa
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Application Notes
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Recommended dilutions. ELISA: 1/5000 - 1/20000. WB: 1/100-1/2000.
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Concentration
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100µg/100µl
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Purification
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Protein G-Sepharose affinity purified
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Buffer
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0.02% Sodium Azide, 50% glycerol, 1% BSA, PBS, pH7.4.
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Storage
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Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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Restrictions
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For Research Use only
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Alternatives for antigen "POU Class 5 Homeobox 1 (POU5F1)", type "Antibodies"
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Hosts
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Rabbit (42), Mouse (15), Goat (7)
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Reactivities
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Human (51), Mouse (Murine) (14), Rat (Rattus) (9), Cow (Bovine) (3), Dog (Canine) (3), Chimpanzee (2), Horse (Equine) (2), Monkey (2), Pig (Porcine) (1), Primate (1)
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Applications
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Western Blotting (WB) (50), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (19), Enzyme Immunoassay (EIA) (18), ELISA (10), Immunofluorescence (IF) (5), Immunoprecipitation (IP) (5), Immunohistochemistry (IHC) (3), Immunohistochemistry (Frozen Sections) (IHC (fro)) (2), Dot Blot (Dot) (1), Immunocytochemistry (ICC) (1)
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Epitopes
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N-Term (2), AA 100-200 (1), AA 230-244 (1), E125 (1), transcript variant 1 (1)
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