The Rabbit Polyclonal anti-Transferrin antibody is suitable to detect Transferrin in samples from Pig. It has been validated for WB, IP, IF, EIA, ID, IHC (fro) and DB.
Hyperimmune antisera with strong precipitating activity are selected for fractionation by Salt Precipitation and purification of the IgG fraction by DEAE-chromatography.
Immunogen
Purified Transferrin isolated from pooled Swine serum. Feund’s complete adjuvant is used in the first step of the immunization procedure.
Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Reconstitution
Restore by adding 1.0 mL of sterile distilled water
Concentration
10 mg/mL
Buffer
PBS, pH 7.2 without preservatives and foreign proteins
Preservative
Without preservative
Storage
4 °C/-20 °C
Storage Comment
Store lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. Avoid Repeated thawing and freezing.
Target
Transferrin (TF)
Background
Transferrin is a single polypeptide chain glycoprotein belonging to iron binding family of proteins. It has a molecular weight of 81,5 kDa (porcine). It is synthesised in the liver and consists of two domains each having a high affinity reversible binding site for Fe3+. The iron is transported in blood and interstitial fluids to sites of use and disposal. Iron/transferrin is essential in haemoglobin synthesis and for certain types of cell division. Serum concentration rises in iron deficiency and pregnancy and falls in iron overload, infection and inflammatory conditions.Synonyms: Beta-1 metal-binding globulin, Serotransferrin, Siderophilin