Alkaline Phosphatase antibody

Catalog No. ABIN799743

The Rabbit Polyclonal anti-Alkaline Phosphatase antibody (ABIN799743) specifically detects Alkaline Phosphatase in WB, ELISA and IP. The antibody is reactive with E. coli samples.

Quick Overview for Alkaline Phosphatase antibody (ABIN799743)

Target: Alkaline Phosphatase (ALP)

Reactivity: E. coli

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Alkaline Phosphatase antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Product Details anti-Alkaline Phosphatase Antibody

Specificity: Alkaline Phosphatase [E. coli]. No cross reactivity occurs against the mammalian form of Alkaline Phosphatase

Purification: Delipidated and defibrinated

Immunogen: Alkaline Phosphatase [E. coli].

Type of Immunogen: Purified protein

Application Details

Application Notes: Approved: ELISA (1:20000 - 1:100000), IP, WB

Usage: Suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation and most immunological methods requiring high titer and specificity. This product has been assayed against 1.0 μg of Alkaline Phosphatase [E. coli] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) catalog no. LS-C60884 and ABTS as a substrate for 30 minutes at room temperature.

Comment:

Target Species of Antibody: E. coli

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: deionized water

Concentration: Lot specific

Buffer: Lyophilized, 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze-thaw cycles.

Target Details for Alkaline Phosphatase

Target: Alkaline Phosphatase (ALP)

Alternative Name: Alkaline Phosphatase