CD16 (GRM1) antibody

Antigen: CD16 (GRM1)

Clonality: Monoclonal (3G8)

Host: Mouse

Reactivity: Human, Primate

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

Catalog no.: ABIN807922

Additional Information

Alternative name: CD16

Gene ID: 2214

Immunogen: Human neutrophils

Isotype: IgG1

Clone: 3G8

Specificity: The mouse monoclonal antibody 3G8 recognizes CD16, a low affinity receptor for aggregated IgG (FcgammaRIII antigen). CD16 exists in two different isoforms: CD16a (FcgammaRIIIA, 50-65 kDa, expressed on NK-cells, monocytes and macrophages) and CD16b (FcgammaRIIIB, 48 kDa, mainly expressed on neutrophils).

Comments:

CD Antigens & MHC (Veterinary), CD and Related Antigens (Human)

Application Details

Buffer: The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.

Storage: Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label. Short-term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.

Research Area: Stem Cells, Hematopoietic Progenitors, Hematopoietic Stem Cells, CD Antigens, Surface Receptors of Immune Cells

Publications

Metes, Ernst, Chambers et al.: "Expression of functional CD32 molecules on human NK cells is determined by an allelic polymorphism of the FcgammaRIIC gene." in: Blood, Vol. 91, Issue 7, pp. 2369-80, 1998 (PubMed).

Zhu, Hamann, Muñoz et al.: "Intracellular expression of Fc gamma RIII (CD16) and its mobilization by chemoattractants in human eosinophils." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 5, pp. 2574-9, 1998 (PubMed).

Komano, Nanki, Hayashida et al.: "Identification of a human peripheral blood monocyte subset that differentiates into osteoclasts." in: Arthritis research & therapy, Vol. 8, Issue 5, pp. R152, 2007 (PubMed).

Choi, Wang, Peterson et al.: "Use of an anti-CD16 antibody for in vivo depletion of natural killer cells in rhesus macaques." in: Immunology, Vol. 124, Issue 2, pp. 215-22, 2008 (PubMed).

Congy-Jolivet, Bolzec, Ternant et al.: "Fc gamma RIIIa expression is not increased on natural killer cells expressing the Fc gamma RIIIa-158V allotype." in: Cancer research, Vol. 68, Issue 4, pp. 976-80, 2008 (PubMed).

Burt, Plitas, Zhao et al.: "The lytic potential of human liver NK cells is restricted by their limited expression of inhibitory killer Ig-like receptors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 183, Issue 3, pp. 1789-96, 2009 (PubMed).