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Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (AA 329-530) antibody

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AA 329-530
Cow (Bovine), Human, Mouse (Murine), Rat (Rattus)
Clonality (Clone)
Monoclonal ()
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
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Immunogen Recombinant fragment corresponding to amino acids 329-530
Clone ESEE122
Specificity Detects ~116 kDa. Specific for HIF1Alpha.
Sensitivity 1 µg/mL of SMC-184 was sufficient for detection of HIF1alpha in 20 µg of CoCl2-induced Hela cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Purification Protein G Purified
Background Hypoxia-inducible factor 1 (HIF1) is a heterodimeric transcription factor that plays a critical role in the cellular response of hypoxia (1). The HIF1 complex consists of two subunits, HIF1-Alpha and HIF1-Beta, which are basic helix-loop-helix proteins of the PAS family (2). HIF1 regulates the transcription of a broad range of genes that facilitate responses to the hypoxic environment, including genes regulating angiogenesis, erythropoiesis, cell cycle, metabolism and apoptosis. The widely expressed HIF-1α is typically degraded rapidly in normoxic cells by the ubiquitin/proteasomal pathway. Under normoxic conditions, HIF-1α is proline hydroxylated leading to a conformational change that promotes binding to the von Hippel Lindau protein (VLH) E3 ligase complex, ubiquitination and proteasomal degradation follows (3, 4). Both hypoxic conditions and chemical hydroxylase inhibitors (such as desferrioxamine and cobalt) inhibit HIF-1α degradation and lead to its stabilization. In addition, HIF-1α can be induced in an oxygen-independent manner by various cytokines through the PI3K-AKT-mTOR pathway (5-7).
Cellular Localization: Cytoplasm | Nucleus
Gene ID 15251
NCBI Accession NP_034561
UniProt Q61221
Application Notes Recommended Dilution: WB (1:1000), IHC (1:100), ICC/IF (1:50), optimal dilutions for assays should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
 image for anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (AA 329-530) antibody (ABIN863096) HIF1alpha (EP190b), Mosue backskin.
Western Blotting (WB) image for anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (AA 329-530) antibody (ABIN863096) Hif1alpha WB 1 in 500 in Hela 20ug untreated
Product cited in: Surazynski, Miltyk, Prokop et al.: "The effect of estrogen on prolidase-dependent regulation of HIF-1α expression in breast cancer cells." in: Molecular and cellular biochemistry, Vol. 379, Issue 1-2, pp. 29-36, 2013 (PubMed).

Background publications Sharp, Bernaudin: "HIF1 and oxygen sensing in the brain." in: Nature reviews. Neuroscience, Vol. 5, Issue 6, pp. 437-48, 2004 (PubMed).

Fukuda, Hirota, Fan et al.: "Insulin-like growth factor 1 induces hypoxia-inducible factor 1-mediated vascular endothelial growth factor expression, which is dependent on MAP kinase and phosphatidylinositol 3-kinase signaling in ..." in: The Journal of biological chemistry, Vol. 277, Issue 41, pp. 38205-11, 2002 (PubMed).

Snyder, Olson, Thomas: "Serum and glucocorticoid-regulated kinase modulates Nedd4-2-mediated inhibition of the epithelial Na+ channel." in: The Journal of biological chemistry, Vol. 277, Issue 1, pp. 5-8, 2002 (PubMed).

Jiang, Jiang, Zheng et al.: "Phosphatidylinositol 3-kinase signaling controls levels of hypoxia-inducible factor 1." in: Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research, Vol. 12, Issue 7, pp. 363-9, 2001 (PubMed).

Laughner, Taghavi, Chiles et al.: "HER2 (neu) signaling increases the rate of hypoxia-inducible factor 1alpha (HIF-1alpha) synthesis: novel mechanism for HIF-1-mediated vascular endothelial growth factor expression." in: Molecular and cellular biology, Vol. 21, Issue 12, pp. 3995-4004, 2001 (PubMed).

Jaakkola, Mole, Tian et al.: "Targeting of HIF-alpha to the von Hippel-Lindau ubiquitylation complex by O2-regulated prolyl hydroxylation." in: Science (New York, N.Y.), Vol. 292, Issue 5516, pp. 468-72, 2001 (PubMed).

Maxwell, Wiesener, Chang et al.: "The tumour suppressor protein VHL targets hypoxia-inducible factors for oxygen-dependent proteolysis." in: Nature, Vol. 399, Issue 6733, pp. 271-5, 1999 (PubMed).

Wang, Jiang, Rue et al.: "Hypoxia-inducible factor 1 is a basic-helix-loop-helix-PAS heterodimer regulated by cellular O2 tension." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 12, pp. 5510-4, 1995 (PubMed).