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HIF1A antibody (AA 728-757)

Name: HIF1A antibody (AA 728-757)
SKU: ABIN3031282
Price: 649.42 USD
Availability: InStock

HIF1A Reactivity: Human, Mouse WB, IHC, ELISA, IF Host: Rabbit Polyclonal unconjugated

Western blot analysis of HIF-1 alpha antibody and MDA-MB231 lysate (HIF1A antibody (AA 728-757))

Confocal immunofluorescent analysis of HIF-1 alpha antibody with MDA-MB231 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). (HIF1A antibody (AA 728-757))

Western blot analysis of HIF-1 alpha antibody and mouse cerebellum tissue lysate (HIF1A antibody (AA 728-757))

IHC analysis of FFPE human hepatocarcinoma tissue stained with HIF-1 alpha antibody (HIF1A antibody (AA 728-757))

IHC

6 images

Catalog No. ABIN3031282

Datasheet as PDF

Target See all HIF1A Antibodies

HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))
Binding Specificity
20

16

16

14

8

7

6

6

6

5

4

4

4

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 728-757
Reactivity
157

92

85

23

23

20

13

11

9

8

7

6

6

5

4

2

2

2

2

2

1

1

1

1

1

1
Human, Mouse
Host
151

49

5
Rabbit
Compatible Secondaries
Clonality
157

48
Polyclonal
Conjugate
103

24

16

6

4

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1
This HIF1A antibody is un-conjugated
Application
165

75

60

52

47

39

39

26

26

25

12

8

7

7

3

3

3

2

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Purification

Purified

Immunogen

A portion of amino acids 728-757 from the human protein was used as the immunogen for this HIF-1 alpha antibody.

Isotype

Ig Fraction

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anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (AA 703-732), (C-Term) antibody
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anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (AA 218-506) antibody
HIF1A Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (AA 575-826) antibody
HIF1A Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (AA 519-547) antibody
HIF1A Reactivity: Human, Mouse WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB14548 unconjugated

anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) antibody
HIF1A Reactivity: Human WB, IHC, ELISA, ICC Host: Mouse Monoclonal 1A3 unconjugated

anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (AA 216-336) antibody
HIF1A Reactivity: Rat WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (AA 519-547) antibody
HIF1A Reactivity: Human, Mouse WB, IHC, ELISA, IF, FACS Host: Rabbit Polyclonal unconjugated

anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (AA 703-732) antibody
HIF1A Reactivity: Human, Mouse, Rat WB, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (AA 329-530) antibody
HIF1A Reactivity: Human, Mouse, Rat, Cow, Dog WB, IHC, IP, IF, ICC Host: Mouse Monoclonal ESEE122 unconjugated

Application Notes

Titration of the HIF-1 alpha antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,Immunofluorescence: 1:10-1:50,IHC (Paraffin): 1:10-1:50

Restrictions

For Research Use only
Format

Liquid

Buffer

In 1X PBS pH 7.4 with 0.09 % sodium azide

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C

Storage Comment

Aliquot the HIF-1 alpha antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Target

HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))

Alternative Name

HIF-1 alpha (HIF1A Products)

Background

Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT).

UniProt

Q16665

Pathways

Positive Regulation of Peptide Hormone Secretion, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Transition Metal Ion Homeostasis, Tube Formation, Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals, Warburg Effect