ATP6V1F antibody

Catalog No. ABIN2716463

This Mouse Monoclonal antibody specifically detects ATP6V1F in WB, IHC, FACS and IF. It exhibits reactivity toward Human.

Quick Overview for ATP6V1F antibody (ABIN2716463)

Target: ATP6V1F (ATPase, H+ Transporting, Lysosomal 14kDa, V1 Subunit F (ATP6V1F))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ATP6V1F antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: 1B8

Product Details anti-ATP6V1F Antibody

Characteristics: Homo sapiens ATPase, H+ transporting, lysosomal 14 kDa, V1 subunit F (ATP6V1F), transcript variant 1

Purification: Purified from mouse ascites fluids by affinity chromatography

Immunogen: Full length human recombinant protein of human ATP6V1F (NP_004222) produced in E.coli.

Isotype: IgG2a

Application Details

Application Notes: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100,

Comment:

The concentration of the product may vary between diferrent lots.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5-1.0 mg/mL

Buffer: PBS (PH 7.3) containing 1 % BSA, 50 % glycerol and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details for ATP6V1F

Target: ATP6V1F (ATPase, H+ Transporting, Lysosomal 14kDa, V1 Subunit F (ATP6V1F))

Alternative Name: ATP6V1F

Background: This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is the V1 domain F subunit protein.

Molecular Weight: 13.2 kDa

Gene ID: 9296

NCBI Accession: NM_004231

HGNC: 9296

Pathways: Transition Metal Ion Homeostasis, Proton Transport