eEF1A1 antibody (AA 131-230) (AbBy Fluor® 647)

Catalog No. ABIN894981

Cited in 1 publication. The AbBy Fluor® 647-conjugated Rabbit Polyclonal anti-eEF1A1 antibody (ABIN894981) specifically detects eEF1A1 in WB, IF (cc) and IF (p). The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for eEF1A1 antibody (AA 131-230) (AbBy Fluor® 647) (ABIN894981)

Target: eEF1A1 (EEF1A1) (Eukaryotic Translation Elongation Factor 1 alpha 1 (EEF1A1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This eEF1A1 antibody is conjugated to AbBy Fluor® 647

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-eEF1A1 Antibody (AbBy Fluor® 647)

Binding Specificity: AA 131-230

Specificity: May crossreact with EEF1A1P5 due to high sequence similarity.

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Pig,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human eEF1A1

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-eEF1A1 antibody (AbBy Fluor® 647) (ABIN894981)

Oh, Lee, Ko, Ko, Kim, Wee: "Analysis of macrophage phenotype in rejected corneal allografts." in: Investigative ophthalmology & visual science, Vol. 54, Issue 12, pp. 7779-84, (2013) (PubMed).

Target Details for eEF1A1

Target: eEF1A1 (EEF1A1) (Eukaryotic Translation Elongation Factor 1 alpha 1 (EEF1A1))

Alternative Name: Eef1a1

Background:

Synonyms: CCS3, EF1A, PTI1, CCS-3, EE1A1, EEF-1, EEF1A, EF-Tu, LENG7, eEF1A-1, GRAF-1EF, HNGC:16303, Elongation factor 1-alpha 1, EF-1-alpha-1, Elongation factor Tu, Eukaryotic elongation factor 1 A-1, Leukocyte receptor cluster member 7, EEF1A1

Background: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. With PARP1 and TXK, forms a complex that acts as a T helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production.

Gene ID: 1915

UniProt: P68104