4-Hydroxy-2-Hexenal (4-HHE) antibody

Catalog No. ABIN920668

This Mouse Monoclonal antibody specifically detects 4-Hydroxy-2-Hexenal (4-HHE) in IHC.

Quick Overview for 4-Hydroxy-2-Hexenal (4-HHE) antibody (ABIN920668)

Target: 4-Hydroxy-2-Hexenal (4-HHE)

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC)

Clone: HHE53

Product Details

Specificity: Specific for HHE-modified protein (especially HHE-His Michael adduct)

Purification: Protein A purified.

Immunogen: HHE-modified keyhole-lympet hemocyanine.

Isotype: IgG1 kappa

Application Details

Application Notes: Immunohistochemistry: Recommended antibody concentration is 0.5 - 1.0 µg/mL on paraformaldehyde fixed tissue.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Monoclonal antibody at 100 µg/mL in 10 mM PBS containing 0.1 % NaN3 and protein stabilizer. Prepared as ascite.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details

Target: 4-Hydroxy-2-Hexenal (4-HHE)

Target Type: Chemical

Background: 4-hydroxy-2-alkenal is one of the major lipid peroxidation products, and shows many biological effects such as high toxicity to cells. Among them, 4-hydroxy-2-hexenal (HHE) is an aldehyde formed during peroxidation of n-3 fatty acids such as docosahexaenoic acid. HHE is a highly reactive aldehyde and reacts with histidine residue of protein to form Michael- addition type adducts. This antibody is specific for HHE-histidine Michael adduct (HHE-His) and enables detection of HHE-His in tissue samples.