Myc Tag antibody

Catalog No. ABIN933570

The Mouse Monoclonal anti-Myc Tag antibody (Clone Myc-A7) (ABIN933570) specifically detects Myc Tag in WB, IP, ELISA and DB.

Quick Overview for Myc Tag antibody (ABIN933570)

Target: Myc Tag

Host: Mouse

Clonality: Monoclonal

Conjugate: This Myc Tag antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), ELISA, Dot Blot (DB)

Clone: Myc-A7

Product Details anti-Myc Tag Antibody

Sequence: EQKLISEEDL

Purification: Protein A affinity chromatography

Immunogen: Myc Tag antibody was raised in mouse using EQKLISEEDL (Myc) synthetic peptide conjugated to KLH as the immunogen.

Isotype: IgG1

Application Details

Application Notes: WB: 1:1000-3000
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as a liquid in 10 mM PBS, pH 7.2, with 0.05 % NaN3

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C for several days to weeks. Add 0.1 % NaN3 and store at -20 °C for longer periods.

Target Details for Myc Tag

Target: Myc Tag

Target Type: Tag

Background: A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by SDS-PAGE protein electrophoresis. Synonyms: Monoclonal Myc Tag antibody, anti-Myc Tag antibody, Myc antibody, C-myc antibody, Cmyc antibody.