Glutamate-Ammonia Ligase (GLUL) antibody

Antigen: Glutamate-Ammonia Ligase (GLUL)

Synonyms: glul, MGC64262, GS, GLNS, PIG43, PIG59, Glns, GLUL, MGC128403, xgs, F15E12.14, F15E12_14, GLUTAMINE SYNTHETASE

Clonality: Polyclonal

Host: Chicken

Reactivity: Synechococcus PCC 7942, Synechocystis PCC 6803

Application: Western Blotting (WB)

Catalog no.: ABIN93525

Additional Information

Alternative name: Glutamine synthetase (GlnA)

Immunogen: KLH-conjugated synthetic peptide derived from available bacterial GlnAsequences with perfect conservation in alpha, beta, gamma Proteobacteria,Enterobacteria, Thermotogales, Low GC Gram+, Cyanobacteria (except weakconservation with Trichodesmium thiebautii) including Synechocystis PCC 6803(Q59981)

Format: Liquid

Description: Glutamine synthetase is the key enzyme in the incorporation of mineral nitrogeninto glutamine.

Specificity: Predicted reactivity: alpha, beta, gamma proteobacteria, enterobacteria, thermotogales,euryarchaeotes, crenarchaeotes. Not reactive in: eukaryotic GlnA.

Application Details

Application Notes: Recommended Dilution 1:5000 with standard ECL (WB). Additional Information: Peptide target used to elicit this antibody has a weak, sporadic conservation withGlutamine Synthetase to III, antibody not expected to detect this enzyme. Weakconservation with some Glutaminyl-tRNA synthetase (Glutamine--tRNA ligase)(GLNRS), but antibody not expected to detect this enzyme.

Concentration: 16 µg/µl

Buffer: total IgY in PBS pH 8.0+ 0.02% sodium azide

Storage: store at 4°C, make aliquots to avoid working with a stock. Please, remember tospin tubes briefly prior to opening them to avoid any losses that might occur fromliquid material adhering to the cap or sides of the tubes.

Research Area: Plant/Algal Physiology

Restrictions: For Research Use only

Images

From the left: Synechococcus sp. 7942 whole cell extract ( 0.35 ug chlorophyll loaded), Synechocystis sp. 6803 whole cell extract, overexpressed GlnA protein standard.