22 Kda Lhc-Like Psii Protein (Psbs) antibody

Antigen: 22 Kda Lhc-Like Psii Protein (Psbs)

Clonality: Polyclonal

Host: Chicken

Reactivity: Arabidopsis thaliana, Oat (Avena sativa), Brassica Napus, Barley (Hordeum vulgare), Pinus sylvestris, Populus tremula, Spinach (Spinacia oleracea)

Application: Western Blotting (WB)

Catalog no.: ABIN93535

Additional Information

Immunogen: KLH-conjugated synthetic peptide derived from available di and monocot PsbSsequences, including Arabidopsis thaliana (At1g44575). This sequence is evenconserved in conifers.

Format: Liquid

Description: The 22 kDa PsbS protein of photosystem II functions in the regulation ofphotosynthetic light harvesting. Along with a low thylakoid lumen pH and thepresence of de-epoxidized xanthophylls, PsbS is necessary for photoprotectivethermal dissipation of excess absorbed light energy in plants, measured asnon-photochemical quenching of chlorophyll fluorescence.

Specificity: Predicted reactivity: dicots and monocots, conifers. Not reactive in: Chlamydomonas reinhardtii, Chlorella sp.

Application Details

Application Notes: Recommended Dilution 1: 2000 - 1 : 4000 (WB).

Buffer: total IgY in PBS pH 8.0+ 0.02% sodium azide

Storage: store at 4°C, make aliquots to avoid working with a stock. Please, remember tospin tubes briefly prior to opening them to avoid any losses that might occur fromliquid material adhering to the cap or sides of the tubes.

Research Area: Plant/Algal Physiology

Restrictions: For Research Use only

Images

Anti-PsbS antibody has been tested in immunoblotting using whole thylakoid extracts from - 1-Corn, 2-Wheat, 3-Brassica, 4-Arabidopsis, 5-Spinach, 6-Chlamydomonas, 7-Chlorella, 8-Pine and Popplar (data not presented) Amount of loaded protein extract - 50-

Publications

Publications: Ensminger, Schmidt, Lloyd: "Soil temperature and intermittent frost modulate the rate of recovery of photosynthesis in Scots pine under simulated spring conditions." in: The New phytologist, Vol. 177, Issue 2, pp. 428-42, 2008 (PubMed).