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Exoenzyme S (Exos) antibody

Antigen

Exoenzyme S (Exos)

Clonality Polyclonal
Host

Chicken

Reactivity

Pseudomonas aeruginosa (P. aeruginosa)

Application
Western Blotting (WB)
Catalog no. ABIN93557
Quantity 100 ul
Price 507.00 $   Plus shipping costs $35.00
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Availability Ships within 3 to 5 Business Days

Additional Information

Immunogen amino acids 366 to 453 of PA3841 of ADP-rbosylating enzyme - Exoenzyme Soverexpressed in a GST fusion. Afterwards cleaved with a help of trombin andseparated on a polyacrylamide gel. Gel piece has been used for immunizations.
Format Liquid
Description Exoenzyme S (ExoS) is a toxin directly translocated into eukaryotic cells by thetype III secretory process of Pseudomonas aeruginosa. It is a bi-functional toxinand contain an N-terminal Rho GAP domain that disrupts the actin cytoskeletonand interference of phagocytosis. The C-terminal of ExoS contains anADP-ribosyltransferase domain, which elicits a cytotoxic phenotype in culturedcells.
Specificity Predicted reactivity: Pseudomonas aeruginosa only.

Application Details

Application Notes Recommended Dilution 1: 5000 (WB).
Buffer total IgY in PBS pH 8.0+ 0.02% sodium azide
Storage store at 4°C, make aliquots to avoid working with a stock. Please, remember tospin tubes briefly prior to opening them to avoid any losses that might occur fromliquid material adhering to the cap or sides of the tubes.
Research Area Bacteria
Restrictions For Research Use only