H Protein of Glycine Decarboxylase Complex (GDC) antibody

Antigen: H Protein of Glycine Decarboxylase Complex (GDC)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Arabidopsis thaliana, Spinach (Spinacia oleracea)

Application: Western Blotting (WB)

Catalog no.: ABIN93559

Additional Information

Alternative name: H protein of glycine decarboxylase complex (GDC) (GDC-H)

Immunogen: purified GDC-H protein from Spinacia oleracea

Format: Lyophilized

Description: The Glycine decarboxylase complex (GDC) is abundant in mitochondria of C3leaves and functions in photorespiratory carbon recovery. GDC enzyme canaccount for up to 50% of matrix protein, and is responsible for the most prominentmetabolic activity in the mitochondria of illuminated leaves, photorespiration. GDCis a multienzyme complex composed of four component enzymes, the P-, H-, T-,and L-proteins and is responsible for the conversion of glycine produced in theperoxisome to serine in the mitochondria during photorespiratory cycle. TheH-protein plays a key role as a mobile substrate that commutes between the othersubunits, allowing its lipoic acid “arm” to visit the active sites of the other threecomponents.

Application Details

Application Notes: Recommended Dilution 1:5 000 with standard ECL (WB).

Buffer: total IgG in PBS pH 7.4

Storage: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior toopening them to avoid any losses that might occur from lyophilized materialadhering to the cap or sides of the tubes.

Research Area: Plant/Algal Physiology

Restrictions: For Research Use only

Images

Detection of GDC-H protein in Arabidopsis thaliana total leaf extract. 15 ug of a total protein has been loaded per lane.