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anti-5 bromodeoxyuridine (BrdU) antibody

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Image for anti-5 bromodeoxyuridine (BrdU) antibody
Antigen: 5 bromodeoxyuridine (BrdU)
Clonality: Monoclonal (MOBU-1)
Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunocytochemistry (ICC)
Reactivity: All Species
Host: Mouse
8 references available 8 references available
Quantity: 0.1 mg (1 mg/ml) (Product variants)
Price: 328,12 $   (Plus shipping costs $25 and VAT, Invoice in EUR)
Order number: ABIN93883
Availability: Ships within 1 to 3 Business days
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Additional Information  » anti-5 bromodeoxyuridine (BrdU) antibody

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Antigen 5 bromodeoxyuridine (BrdU)
Immunogen 5-bromodeoxyuridine conjugated with hemocyanine.
Reactivity All Species
Clonality Monoclonal
Format Purified
Isotype IgG1 »Matching secondary antibodies
Description Bromodexyuridine (BrdU) is a thymidine analog which is selectively incorporated into the DNA of proliferating cells to provide a marker for the DNA being replicated. The number of proliferating cells can then be detected in cell lysates, tissue sections or suspensions using an antibody specific for the BrdU.
Clone MOBU-1
Host Mouse
Specificity The antibody MoBu-1 reacts specifically with BrdU incorporated into DNA during S-phase of a cell cycle. The antibody MoBu-1 is also useful for detecting proliferating cells by flow cytometry or immunofluorescence staining. It reacts also specifically with 5-bromouridine (BrU).

Application Details  » anti-5 bromodeoxyuridine (BrdU) antibody

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Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunocytochemistry (ICC)
» Click here to view 6 available protocols!
Application Notes Immunocytochemistry: 2 µg/ml. Works very well for Immunohistochemistry with paraffin embedded sections.
Concentration 1 mg/ml
Purity > 95% (by SDS-PAGE)
Purification Purified from ascites by protein-A affinity chromatography.
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Storage Store at 2-8
Research Area Cell Cycle
Restrictions For Research Use only

Publications  » anti-5 bromodeoxyuridine (BrdU) antibody

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Publications Schielen, Van Der Pijl, Bleumink et al.: "Local popliteal lymph node reactions to hexachlorobenzene and pentachlorobenzene: comparison with systemic effects." In: Immunopharmacology , Vol. 31, Issue 02. Mrz, pp. 171-81, 1997 (PubMed)

Peters, Ganesh, Kuipers et al.: "Epithelial cell proliferative activity of Barrett's esophagus: methodology and correlation with traditional cancer risk markers." In: Digestive diseases and sciences , Vol. 43, Issue 7, pp. 1501-6, 1998 (PubMed)

Buckiovua, Kubuinovua, Soukup et al.: "Hyperthermia in the chick embryo: HSP and possible mechanisms of developmental defects." In: The International journal of developmental biology , Vol. 42, Issue 5, pp. 737-40, 1998 (PubMed)

Stanek, Kiss, Raska: "Pre-ribosomal RNA is processed in permeabilised cells at the site of transcription." In: European journal of cell biology , Vol. 79, Issue 3, pp. 202-7, 2000 (PubMed)

Peters, Ganesh, Kuipers et al.: "Effect of elimination of acid reflux on epithelial cell proliferative activity of Barrett esophagus." In: Scandinavian journal of gastroenterology , Vol. 35, Issue 12, pp. 1238-44, 2001 (PubMed)

Swee, Bosco, Malissen et al.: "Expansion of peripheral naturally occurring T regulatory cells by Fms-like tyrosine kinase 3 ligand treatment." In: Blood , Vol. 113, Issue 25, pp. 6277-87, 2009 (PubMed)

Gamper, Koh, Ruau et al.: "GAR22: a novel target gene of thyroid hormone receptor causes growth inhibition in human erythroid cells." In: Experimental hematology , Vol. 37, Issue 5, pp. 539-548.e4, 2009 (PubMed)

Johnson, Phua, Bennett et al.: "Studying vertebrate topoisomerase 2 function using a conditional knockdown system in DT40 cells." In: Nucleic acids research , Vol. 37, Issue 14, pp. e98, 2009 (PubMed)