Bromodeoxyuridine (BrDU) antibody

Antigen: Bromodeoxyuridine (BrDU)

Clonality: Monoclonal (MoBu-1)

Host: Mouse

Application: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Catalog no.: ABIN93883

Additional Information

Alternative name: 5-bromodeoxyuridine (BrdU)

Immunogen: 5-bromodeoxyuridine conjugated with hemocyanine.

Format: Purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: MoBu-1

Description: Bromodexyuridine (BrdU) is a thymidine analog which is selectively incorporated into theDNA of proliferating cells to provide a marker for the DNA being replicated. The number ofproliferating cells can then be detected in cell lysates, tissue sections or suspensions usingan antibody specific for the BrdU.

Specificity: The antibody MoBu-1 reacts specifically with BrdU incorporated into DNA during S-phase ofa cell cycle. The antibody MoBu-1 is also useful for detecting proliferating cells by flowcytometry or immunofluorescence staining. It reacts also specifically with 5-bromouridine(BrU).

Application Details

Application Notes: Immunohistochemistry (paraffin sections). Application note: excellent. Immunocytochemistry - Recommended dilution: 2 µg/ml

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from ascites by protein-A affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Restrictions: For Research Use only

Images

Flow Cytometry analysis of 5-bromodeoxyuridin (BrdU) incorporation in CEM (human acute lymphoblastic leukemia cell line) using 5-bromodeoxyuridine (BrdU) monoclonal antibody, clone MoBu-1 (ABIN93883) (detection by Goat anti-mouse IgG1 FITC). The individual cell cycle phases (S-, G1-, G2/M-phase) are indicated in the figure.

Publications

Publications: Schielen, Van Der Pijl, Bleumink et al.: "Local popliteal lymph node reactions to hexachlorobenzene and pentachlorobenzene: comparison with systemic effects." in: Immunopharmacology, Vol. 31, Issue 02. Mrz, pp. 171-81, 1997 (PubMed).

Peters, Ganesh, Kuipers et al.: "Epithelial cell proliferative activity of Barrett's esophagus: methodology and correlation with traditional cancer risk markers." in: Digestive diseases and sciences, Vol. 43, Issue 7, pp. 1501-6, 1998 (PubMed).

Buckiovua, Kubuinovua, Soukup et al.: "Hyperthermia in the chick embryo: HSP and possible mechanisms of developmental defects." in: The International journal of developmental biology, Vol. 42, Issue 5, pp. 737-40, 1998 (PubMed).

Stanek, Kiss, Raska: "Pre-ribosomal RNA is processed in permeabilised cells at the site of transcription." in: European journal of cell biology, Vol. 79, Issue 3, pp. 202-7, 2000 (PubMed).

Peters, Ganesh, Kuipers et al.: "Effect of elimination of acid reflux on epithelial cell proliferative activity of Barrett esophagus." in: Scandinavian journal of gastroenterology, Vol. 35, Issue 12, pp. 1238-44, 2001 (PubMed).

Swee, Bosco, Malissen et al.: "Expansion of peripheral naturally occurring T regulatory cells by Fms-like tyrosine kinase 3 ligand treatment." in: Blood, Vol. 113, Issue 25, pp. 6277-87, 2009 (PubMed).

Gamper, Koh, Ruau et al.: "GAR22: a novel target gene of thyroid hormone receptor causes growth inhibition in human erythroid cells." in: Experimental hematology, Vol. 37, Issue 5, pp. 539-548.e4, 2009 (PubMed).

Johnson, Phua, Bennett et al.: "Studying vertebrate topoisomerase 2 function using a conditional knockdown system in DT40 cells." in: Nucleic acids research, Vol. 37, Issue 14, pp. e98, 2009 (PubMed).

Babic, Ondrejcakova, Bakos et al.: "Cell proliferation in the hippocampus and in the heart is modified by exposure to repeated stress and treatment with memantine." in: Journal of psychiatric research, Vol. 46, Issue 4, pp. 526-32, 2012 (PubMed). Method employed by authors: Immunohistochemistry (IHC) (1:500). Further details: The sections were incubated with mouse monoclonal anti-BrdU antibody (1:500) at 4 degrees Celsius overnight.