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Antibody
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anti-alpha-Fetoprotein AFP
alpha-Fetoprotein (AFP) antibody
| Antigen |
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| Synonyms |
FETA, HPAFP, AFP, MGC128267 |
| Clonality |
Monoclonal (AFP-11) |
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Host
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Reactivity
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Human (170), Mouse (Murine) (31), Dog (Canine) (21), Pig (Porcine) (21), Rat (Rattus) (19), Cow (Bovine) (15), Horse (Equine) (15), Rabbit (15), Mammalian (1), Monkey (1), Sheep (Ovine) (1)
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Application
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 Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Radioimmunoassay (RIA)
Western Blotting (WB) (61), Enzyme Immunoassay (EIA) (55), ELISA (50), Immunofluorescence (IF) (41), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (36), Flow Cytometry (FACS) (35), Immunohistochemistry (Frozen Sections) (IHC (fro)) (20), Immunohistochemistry (IHC) (18), Radioimmunoassay (RIA) (18), Immunoprecipitation (IP) (16), Immunoelectrophoresis (IEP) (5), ELISA (Capture) (4), ELISA (Detection) (4), Immunodiffusion (ID) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), Lateral Flow (LF) (3), Immunocytochemistry (ICC) (2), Immunoelectron Microscopy (EM) (2), Radial Immunodiffusion (RID) (2), Conjugation (1), ELISA (Coating) (1), Gel Diffusion (GD) (1), Indirect ELISA (iELISA) (1)
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| Catalog no. |
ABIN93887 |
| Quantity |
0.1 mg (1 mg/ml) |
| Price |
234.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 3 to 5 Business days |
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Alternative name
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alpha-Fetoprotein
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Immunogen
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purified alpha-Fetoprotein
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Format
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Purified
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Isotype
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IgG1 (Matching secondary antibodies)
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Clone
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AFP-11
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Specificity
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The antibody AFP-11 reacts with human alpha-Fetoprotein (AFP), a 70 kDa oncofetalantigen. AFP is a major fetal plasma protein, but is not present in healthy adult tissues.Elevated AFP concentrations in adult plasma may be an early marker of hepatocellularcarcinoma or teratoblastoma, while high concentrations in amniotic fluid may indicate severecongenital defects of a fetus.
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Application Notes
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Immunohistochemistry (paraffin sections) - Recommended dilution: 10 µg/ml. Positive tissue: hepatocellular carcinoma. Application note: heat mediated antigen retrieval (sodium citrate buffer), mAb incubation 1hour / RT, detection DABELISA: The antibody AFP-11 has been tested as the capture antibody in a sandwich ELISA for analysis of human alpha-Fetoprotein in combination with antibody AFP-01 (cat. no. 11-259-C100).
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Concentration
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1 mg/ml
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Purity
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> 95% (by SDS-PAGE)
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Purification
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Purified from ascites by precipitation methods.
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Buffer
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Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
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Storage
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Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.
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Research Area
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Serum/Plasma Proteins
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Restrictions
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For Research Use only
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Publications
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Stefanovua, Horejsui, Kristofovua et al.: "Monoclonal antibodies against human alpha-fetoprotein. Exploitation of an unusual calcium-dependent interaction with the antigen for analytical and preparative purposes." in: Journal of immunological methods, Vol. 111, Issue 1, pp. 67-73, 1988 (PubMed).
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Alternatives for antigen "alpha-Fetoprotein (AFP)", type "Antibodies"
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Hosts
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Mouse (111), Rabbit (72), Goat (10), Sheep (3), Duck (2)
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Reactivities
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Human (170), Mouse (Murine) (31), Dog (Canine) (21), Pig (Porcine) (21), Rat (Rattus) (19), Cow (Bovine) (15), Horse (Equine) (15), Rabbit (15), Mammalian (1), Monkey (1), Sheep (Ovine) (1)
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Applications
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Western Blotting (WB) (61), Enzyme Immunoassay (EIA) (55), ELISA (50), Immunofluorescence (IF) (41), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (36), Flow Cytometry (FACS) (35), Immunohistochemistry (Frozen Sections) (IHC (fro)) (20), Immunohistochemistry (IHC) (18), Radioimmunoassay (RIA) (18), Immunoprecipitation (IP) (16), Immunoelectrophoresis (IEP) (5), ELISA (Capture) (4), ELISA (Detection) (4), Immunodiffusion (ID) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), Lateral Flow (LF) (3), Immunocytochemistry (ICC) (2), Immunoelectron Microscopy (EM) (2), Radial Immunodiffusion (RID) (2), Conjugation (1), ELISA (Coating) (1), Gel Diffusion (GD) (1), Indirect ELISA (iELISA) (1)
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Conjugates
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HRP (6), Biotin (5), FITC (4), Alexa Flour 488 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), Gold (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy7 (2), RBITC (2)
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Epitopes
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N-Term (3), a (2), Center (1), b (1), c (1)
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