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Antibodies
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anti-alpha-Fetoprotein (AFP) antibody
alpha-Fetoprotein (AFP) antibody
| Antigen |
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| Synonyms |
FETA, HPAFP, AFP, MGC128267 |
| Clonality |
Monoclonal (AFP-01) |
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Host
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Reactivity
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Human (193), Mouse (Murine) (46), Rat (Rattus) (31), Dog (Canine) (28), Pig (Porcine) (24), Cow (Bovine) (23), Horse (Equine) (17), Rabbit (17), Chicken (5), Sheep (Ovine) (4), Monkey (3), Fruit Fly (Drosophila melanogaster) (1), Mammalian (1), Zebrafish (Brachydanio rerio) (1)
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Application
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 Alternatives Immunoprecipitation (IP), Western Blotting (WB), ELISA, Radioimmunoassay (RIA)
Western Blotting (WB) (82), Immunofluorescence (IF) (66), Flow Cytometry (FACS) (65), Enzyme Immunoassay (EIA) (55), ELISA (52), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (37), Immunohistochemistry (IHC) (21), Immunohistochemistry (Frozen Sections) (IHC (fro)) (20), Radioimmunoassay (RIA) (18), Immunoprecipitation (IP) (15), ELISA (Capture) (5), ELISA (Detection) (5), Immunoelectrophoresis (IEP) (5), Lateral Flow (LF) (5), Immunodiffusion (ID) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), To be determined by user (TBD) (4), Immunocytochemistry (ICC) (2), Immunoelectron Microscopy (IEM) (2), Radial Immunodiffusion (RID) (2), Conjugation (1), ELISA (Coating) (1), Gel Diffusion (GD) (1), Indirect ELISA (iELISA) (1)
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| Catalog no. |
ABIN93889 |
| Quantity |
0.1 mg (1 mg/ml) |
| Price |
234.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 3 to 5 Business Days |
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Alternative name
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alpha-Fetoprotein
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Immunogen
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purified alpha-Fetoprotein
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Format
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Purified
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Isotype
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IgG1 (Matching secondary antibodies)
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Clone
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AFP-01
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Specificity
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The antibody AFP-01 reacts with human alpha-Fetoprotein (AFP), a 70 kDa oncofetalantigen. AFP is a major fetal plasma protein, but is not present in healthy adult tissues.Elevated AFP concentrations in adult plasma may be an early marker of hepatocellularcarcinoma or teratoblastoma, while high concentrations in amniotic fluid may indicate severecongenital defects of a fetus.
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Application Notes
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Immunoprecipitation. Application note: Interaction of the antibody AFP-01 with AFP is dependent on the presenceof calcium ions (strongly inhibited by chelating agents). Such characteristics of the antibodycan be exploited for immunoaffinity purification of APF under mild elution conditions. ELISA: The antibody AFP-01 has been tested as the detection antibody in a sandwich ELISA for analysis of human alpha-Fetoprotein in combination with antibody AFP-11 (cat. no. 11-384-C100).Positive material: Hep G2 human hepatocellular carcinoma cell line. Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1%laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8. 2, 50 mM NaF including Proteaseinhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate withnon-reducing/reducing Laemmli SDS-PAGE sample buffer. Application note: Both non-reducing and reducing conditions.
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Concentration
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1 mg/ml
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Purity
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> 95% (by SDS-PAGE)
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Purification
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Purified from ascites by precipitation methods.
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Buffer
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Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
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Storage
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Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.
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Research Area
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Serum/Plasma Proteins
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Restrictions
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For Research Use only
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Alternatives for antigen "alpha-Fetoprotein (AFP)", type "Antibodies"
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Hosts
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Mouse (145), Rabbit (83), Goat (14), Sheep (3), Duck (2)
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Reactivities
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Human (193), Mouse (Murine) (46), Rat (Rattus) (31), Dog (Canine) (28), Pig (Porcine) (24), Cow (Bovine) (23), Horse (Equine) (17), Rabbit (17), Chicken (5), Sheep (Ovine) (4), Monkey (3), Fruit Fly (Drosophila melanogaster) (1), Mammalian (1), Zebrafish (Brachydanio rerio) (1)
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Applications
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Western Blotting (WB) (82), Immunofluorescence (IF) (66), Flow Cytometry (FACS) (65), Enzyme Immunoassay (EIA) (55), ELISA (52), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (37), Immunohistochemistry (IHC) (21), Immunohistochemistry (Frozen Sections) (IHC (fro)) (20), Radioimmunoassay (RIA) (18), Immunoprecipitation (IP) (15), ELISA (Capture) (5), ELISA (Detection) (5), Immunoelectrophoresis (IEP) (5), Lateral Flow (LF) (5), Immunodiffusion (ID) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), To be determined by user (TBD) (4), Immunocytochemistry (ICC) (2), Immunoelectron Microscopy (IEM) (2), Radial Immunodiffusion (RID) (2), Conjugation (1), ELISA (Coating) (1), Gel Diffusion (GD) (1), Indirect ELISA (iELISA) (1)
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Conjugates
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HRP (7), Biotin (6), Alexa Fluor 350 (4), Alexa Fluor 488 (4), Alexa Fluor 647 (4), FITC (4), PE,Cy5.5 (4), Alexa Fluor 555 (3), Alexa Flour 488 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), Gold (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy7 (2), RBITC (2)
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Epitopes
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N-Term (3), a (2), Center (1), b (1), c (1)
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