Tubulin, Beta, 3 (TUBB3) antibody

Antigen: Tubulin, Beta, 3 (TUBB3)

Synonyms: TUBB3, TUBB4, MGC134115, MC1R, beta-4, tbb3, M(beta)3, M(beta)6, 3200002H15Rik, tubb3, MGC76202, TUBB1, tubB3, tubulin

Clonality: Monoclonal (TU-20)

Host: Mouse

Reactivity: Various Species

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Radioimmunoassay (RIA), Immunocytochemistry (ICC)

Catalog no.: ABIN93911

Additional Information

Alternative name: betaIII-tubulin

Immunogen: Peptide (C) 441-448 coupled to maleimide-activated keyhole limpet hemocyanin viacysteine added to the N-terminus of the neuron-specific peptide.

Format: Purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: TU-20

Description: The betaIII-tubulin isoform is present dominantly in cells of neuronal origin and it is one ofthe earliest markers of neuronal differentiation. It is regarded as a specific probe for the cellsof neuronal origin as well as for the tumours originating from these cells. The betaIII-tubulinis most abundant in cells of neuronal origin, but was also detected in Sertoli cells of thetestis and transiently in non-neuronal embryonic tissues.

Specificity: The antibody TU-20 recognizes C-terminal peptide sequence ESESQGPK (aa 441-448) ofneuron-specific human betaIII-tubulin.

Application Details

Application Notes: Western Blotting - Recommended dilution: 1-2 µg/ml, 90 min Positive control: Porcine brain lysate. Negative control: HPB-ALL human peripheral blood leukemia cell line. Sample preparation: Mix lysate with reducing Laemmli SDS-PAGE sample buffer. Application note: Reducing conditions. Immunohistochemistry (paraffin sections) - Recommended dilution: 10 µg/ml Staining technique: Standard ABC technique (DAB+)Pretreatment: 0.1% pepsin (trypsin) in 0.1 M HCl, incubation 30 min in RT, or Hightemperature citrate buffer antigen retrieval Positive tissue: neuronal tissue. Immunocytochemistry - Positive material: Neuro2a mouse neuroblastoma cell line

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from ascites by precipitation methods.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Restrictions: For Research Use only

Images

Immunofluorescence staining (mouse neuroblastoma cells)

Publications

Publications: Draberova E, Lukas Z, Ivanyi D et al.: "Expression of class III beta-tubulin in normal and neoplastic human tissues." in: Histochem Cell Biol, Vol. 109, Issue 3, pp. 231-9, 1998 (PubMed).

P?knicova, Kubatova, Sulimenko et al.: "Differential subcellular distribution of tubulin epitopes in boar spermatozoa: recognition of class III beta-tubulin epitope in sperm tail." in: Biology of reproduction, Vol. 65, Issue 3, pp. 672-9, 2001 (PubMed).

Woltjen, Michael, Mohseni et al.: "piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells." in: Nature, Vol. 458, Issue 7239, pp. 766-70, 2009 (PubMed).

Silva, Pereira, Oliveira et al.: "BDNF and extracellular matrix regulate differentiation of mice neurosphere-derived cells into a GABAergic neuronal phenotype." in: Journal of neuroscience research, Vol. 87, Issue 9, pp. 1986-96, 2009 (PubMed).

Theodorou, Dalembert, Heffelfinger et al.: "A high throughput embryonic stem cell screen identifies Oct-2 as a bifunctional regulator of neuronal differentiation." in: Genes & development, Vol. 23, Issue 5, pp. 575-88, 2009 (PubMed).

Kunova, Matulka, Eiselleova et al.: "Development of humanized culture medium with plant-derived serum replacement for human pluripotent stem cells." in: Reproductive biomedicine online, Vol. 21, Issue 5, pp. 676-86, 2010 (PubMed).

Gun?ova: "The neurodegenerative process in a neurotoxic rat model and in patients with Huntington's disease: Histopathological parallels and differences." in: Acta histochemica, 2011 (PubMed).