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|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Tubulin, Beta, 3 (TUBB3) antibody
|Synonyms||TUBB3, TUBB4, MGC134115, MC1R, beta-4, tbb3, M(beta)3, M(beta)6, 3200002H15Rik, tubb3, MGC76202, TUBB1, tubB3, tubulin|
Alternatives Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Radioimmunoassay (RIA), Immunocytochemistry (ICC)
|12 references available|
|Quantity||0.1 mg (1 mg/ml)|
|Price||348.48 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 4 to 5 Business Days|
|Immunogen||Peptide (C) 441-448 coupled to maleimide-activated keyhole limpet hemocyanin viacysteine added to the N-terminus of the neuron-specific peptide.|
|Description||The betaIII-tubulin isoform is present dominantly in cells of neuronal origin and it is one ofthe earliest markers of neuronal differentiation. It is regarded as a specific probe for the cellsof neuronal origin as well as for the tumours originating from these cells. The betaIII-tubulinis most abundant in cells of neuronal origin, but was also detected in Sertoli cells of thetestis and transiently in non-neuronal embryonic tissues.|
|Specificity||The antibody TU-20 recognizes C-terminal peptide sequence ESESQGPK (aa 441-448) ofneuron-specific human betaIII-tubulin.|
|Application Notes||Western Blotting - Recommended dilution: 1-2 µg/ml, 90 min Positive control: Porcine brain lysate. Negative control: HPB-ALL human peripheral blood leukemia cell line. Sample preparation: Mix lysate with reducing Laemmli SDS-PAGE sample buffer. Application note: Reducing conditions. Immunohistochemistry (paraffin sections) - Recommended dilution: 10 µg/ml Staining technique: Standard ABC technique (DAB+)Pretreatment: 0.1% pepsin (trypsin) in 0.1 M HCl, incubation 30 min in RT, or Hightemperature citrate buffer antigen retrieval Positive tissue: neuronal tissue. Immunocytochemistry - Positive material: Neuro2a mouse neuroblastoma cell line|
|Purity||> 95% (by SDS-PAGE)|
|Purification||Purified from ascites by precipitation methods and ion exchange chromatography.|
|Buffer||Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4|
|Storage||Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.|
|Restrictions||For Research Use only|
|Immunofluorescence staining (mouse neuroblastoma cells)|
P?knicova, Kubatova, Sulimenko et al.: "Differential subcellular distribution of tubulin epitopes in boar spermatozoa: recognition of class III beta-tubulin epitope in sperm tail." in: Biology of reproduction, Vol. 65, Issue 3, pp. 672-9, 2001 (PubMed).
Woltjen, Michael, Mohseni et al.: "piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells." in: Nature, Vol. 458, Issue 7239, pp. 766-70, 2009 (PubMed).
Silva, Pereira, Oliveira et al.: "BDNF and extracellular matrix regulate differentiation of mice neurosphere-derived cells into a GABAergic neuronal phenotype." in: Journal of neuroscience research, Vol. 87, Issue 9, pp. 1986-96, 2009 (PubMed).
Theodorou, Dalembert, Heffelfinger et al.: "A high throughput embryonic stem cell screen identifies Oct-2 as a bifunctional regulator of neuronal differentiation." in: Genes & development, Vol. 23, Issue 5, pp. 575-88, 2009 (PubMed).
Kunova, Matulka, Eiselleova et al.: "Development of humanized culture medium with plant-derived serum replacement for human pluripotent stem cells." in: Reproductive biomedicine online, Vol. 21, Issue 5, pp. 676-86, 2010 (PubMed).
Gun?ova: "The neurodegenerative process in a neurotoxic rat model and in patients with Huntington's disease: Histopathological parallels and differences." in: Acta histochemica, 2011 (PubMed).
Dráberová, Lukás, Ivanyi et al.: "Expression of class III beta-tubulin in normal and neoplastic human tissues." in: Histochemistry and cell biology, Vol. 109, Issue 3, pp. 231-9, 1998 (PubMed).
Zíková, Sulimenko, Dráber et al.: "Accumulation of 210 kDa microtubule-interacting protein in differentiating P19 embryonal carcinoma cells." in: FEBS letters, Vol. 473, Issue 1, pp. 19-23, 2000 (PubMed).
Kukharskyy, Sulimenko, Macůrek et al.: "Complexes of gamma-tubulin with nonreceptor protein tyrosine kinases Src and Fyn in differentiating P19 embryonal carcinoma cells." in: Experimental cell research, Vol. 298, Issue 1, pp. 218-28, 2004 (PubMed).
Katsetos, Dráberová, Smejkalová et al.: "Class III beta-tubulin and gamma-tubulin are co-expressed and form complexes in human glioblastoma cells." in: Neurochemical research, Vol. 32, Issue 8, pp. 1387-98, 2007 (PubMed).
Dráberová, Del Valle, Gordon et al.: "Class III beta-tubulin is constitutively coexpressed with glial fibrillary acidic protein and nestin in midgestational human fetal astrocytes: implications for phenotypic identity." in: Journal of neuropathology and experimental neurology, Vol. 67, Issue 4, pp. 341-54, 2008 (PubMed).
Katsetos, Draber, Kavallaris: "Targeting βIII-tubulin in glioblastoma multiforme: from cell biology and histopathology to cancer therapeutics." in: Anti-cancer agents in medicinal chemistry, Vol. 11, Issue 8, pp. 719-28, 2012 (PubMed).
|Hosts||Mouse (19), Rabbit (12), Goat (3), Chicken (2)|
|Reactivities||Human (25), Rat (Rattus) (12), Mouse (Murine) (10), Cow (Bovine) (3), General (2), Pig (Porcine) (2), Various Species (2), Rabbit (1)|
|Applications||Western Blotting (WB) (28), Immunofluorescence (IF) (18), ELISA (13), Immunohistochemistry (IHC) (10), Flow Cytometry (FACS) (9), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (8), Immunoprecipitation (IP) (5), Immunocytochemistry (ICC) (4)|
|Conjugates||FITC (3), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Dyomics 547 (1), PE,Cy5.5 (1)|
|Epitopes||pSer172 (4), N-Term (3), C-Term (2), AA 436-450 (1), Internal Region,N-Term (1), pThr417 (1)|