Tubulin, Beta, 3 (TUBB3) antibody (FITC)

Antigen: Tubulin, Beta, 3 (TUBB3)

Synonyms: TUBB3, TUBB4, MGC134115, MC1R, beta-4, tbb3, M(beta)3, M(beta)6, 3200002H15Rik, tubb3, MGC76202, TUBB1, tubB3, tubulin

Clonality: Monoclonal (TU-20)

Host: Mouse

Reactivity: Various Species

Conjugate: FITC

Catalog no.: ABIN93912

Additional Information

Alternative name: betaIII-tubulin

Immunogen: Peptide (C) 441-448 coupled to maleimide-activated keyhole limpet hemocyanin viacysteine added to the N-terminus of the neuron-specific peptide.

Isotype: IgG1

Clone: TU-20

Description: The betaIII-tubulin isoform is present dominantly in cells of neuronal origin and it is one ofthe earliest markers of neuronal differentiation. It is regarded as a specific probe for the cellsof neuronal origin as well as for the tumours originating from these cells. The betaIII-tubulinis most abundant in cells of neuronal origin, but was also detected in Sertoli cells of thetestis and transiently in non-neuronal embryonic tissues.

Specificity: The antibody TU-20 recognizes C-terminal peptide sequence ESESQGPK (aa 441-448) ofneuron-specific human betaIII-tubulin.

Application Details

Application Notes: Immunocytochemistry on fixed and permeabilized cells. Suggested working dilution is 1: 40.The conjugate was also successfully used on paraffin sections using confocal microscopy. It is recommended that the user titrates the reagent for use in the particular testing system.

Concentration: 1.0 mg/ml

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store in the dark at 2-8 o C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.Short-term exposure to room temperature should not affect the quality of the reagent.However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.

Restrictions: For Research Use only

Images

Immunofluorescence staining (mouse neuroblastoma cells)

Publications

Publications: Draberova E, Lukas Z, Ivanyi D et al.: "Expression of class III beta-tubulin in normal and neoplastic human tissues." in: Histochem Cell Biol, Vol. 109, Issue 3, pp. 231-9, 1998 (PubMed).

P?knicova, Kubatova, Sulimenko et al.: "Differential subcellular distribution of tubulin epitopes in boar spermatozoa: recognition of class III beta-tubulin epitope in sperm tail." in: Biology of reproduction, Vol. 65, Issue 3, pp. 672-9, 2001 (PubMed).