Blood Group Lewis A antibody

Antigen: Blood Group Lewis A

Clonality: Monoclonal (7LE)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA

Catalog no.: ABIN93924

Additional Information

Immunogen: Mucin isolated by size-exclusion chromatography from an ovarian cyst fluid (pureendocervical type according to the Fenoglio's classification).

Format: Purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: 7LE

Specificity: The antibody 7LE recognizes Lewis a blood group antigen, a carbohydrate determinant carried on both glycolipids and glycoproteins, expressed in colonic epithelial cells. Lewis a may be useful for detection of gastrointestinal, pancreatic and colorectal tumors.

Application Details

Application Notes: Immunohistochemistry (paraffin sections) - Recommended dilution: 5 µg/ml. Positive control: colorectal adenocarcinoma. Application note: heat mediated antigen retrieval (sodium citrate), mAb incubation 1 hour /RT, detection DAB

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from hybridoma culture supernatant by protein-A affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Restrictions: For Research Use only

Images

Immunohistochemistry staining of colorectal adenocarcinoma (paraffin-embedded sections) with anti-human Blood Group Lewis a (7LE)

Publications

Publications: Creuzot-Garcher, Guerzider, Assem et al.: "Alteration of sialyl Lewis epitope expression in pterygium." in: Investigative ophthalmology & visual science, Vol. 40, Issue 8, pp. 1631-6, 1999 (PubMed).