Fc Fragment of IgG, Low Affinity IIIa, Receptor (CD16a) (FCGR3A) antibody

Antigen: Fc Fragment of IgG, Low Affinity IIIa, Receptor (CD16a) (FCGR3A)

Synonyms: CD16, FCG3, CD16A, FCGR3, IGFR3, FCR-10, FCRIII, FCGRIII, FCRIIIA

Clonality: Monoclonal (LNK16)

Host: Mouse

Reactivity: Human, Primates

Application: Flow Cytometry (FACS), Immunoprecipitation (IP)

Catalog no.: ABIN93988

Additional Information

Alternative name: CD16

Immunogen: Normal human peripheral blood granulocytes

Format: Purified

Isotype: IgG1

Clone: LNK16

Description: CD16 (FcgammaRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor.Human FcgammaRIII is expressed in two forms FcgammaRIII-A and -B. FcgammaRIII-A isa transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It isassociated with FcepsilonRI-gamma subunit and is responsible for antibody-dependent NKcell cytotoxicity. Mast cell FcgammaRIII-A is associated, moreover, with FcepsilonRI-betasubunit. Besides IgG, FcgammaRIII-A can be triggered also by oligomeric IgE.FcgammaRIII-B is a GPI-linked monomeric receptor expressed on neutrophils and isinvolved in their activation and induction of a proadhesive phenotype.

Specificity: The antibody LNK16 reacts with CD16, a low affinity receptor for aggregated IgG(FcgammaRIII antigen). CD16 exists in two different isoforms: CD16a (FcgammaRIIIA,50-65 kDa, expressed on NK-cells, monocytes and macrophages) and CD16b(FcgammaRIIIB, 48 kDa, mainly expressed on neutrophils).HLDA V, WS Code M MA069HLDA V, WS Code NK NK50

Application Details

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from ascites by precipitation methods and ion exchange chromatography.

Buffer: Tris buffered saline (TBS) with 15 mM sodium azide, approx. pH 8.0

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Research Area: Stem Cells, Hematopoietic Progenitors, Hematopoietic Stem Cells, CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only

Images

Flow Cytometry analysis

Publications

Publications: Mathison, Befus, Davison et al.: "Modulation of neutrophil function by the tripeptide feG." in: BMC immunology, Vol. 4, pp. 3, 2003 (PubMed).

Lin, Liu, Chen et al.: "CD94 1A transcripts characterize lymphoblastic lymphoma/leukemia of immature natural killer cell origin with distinct clinical features." in: Blood, Vol. 106, Issue 10, pp. 3567-74, 2005 (PubMed).

Congy-Jolivet, Bolzec, Ternant et al.: "Fc gamma RIIIa expression is not increased on natural killer cells expressing the Fc gamma RIIIa-158V allotype." in: Cancer research, Vol. 68, Issue 4, pp. 976-80, 2008 (PubMed).

Mathison, Christie, Davison: "The tripeptide feG inhibits leukocyte adhesion." in: Journal of inflammation (London, England), Vol. 5, pp. 6, 2008 (PubMed).

Chen, Freedman: "CD16+ gammadelta T cells mediate antibody dependent cellular cytotoxicity: potential mechanism in the pathogenesis of multiple sclerosis." in: Clinical immunology (Orlando, Fla.), Vol. 128, Issue 2, pp. 219-27, 2008 (PubMed).

Kahn, Moergelin, Shannon et al.: "Antibodies against a surface protein of Streptococcus pyogenes promote a pathological inflammatory response." in: PLoS pathogens, Vol. 4, Issue 9, pp. e1000149, 2008 (PubMed).

Meknache, Joensson, Laurent et al.: "Human basophils express the glycosylphosphatidylinositol-anchored low-affinity IgG receptor FcgammaRIIIB (CD16B)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 4, pp. 2542-50, 2009 (PubMed).

Mehrle, Watzl, von Lilienfeld-Toal et al.: "Comparison of phenotype of gammadelta T cells generated using various cultivation methods." in: Immunology letters, Vol. 125, Issue 1, pp. 53-8, 2009 (PubMed).