Fc Fragment of IgG, Low Affinity IIIa, Receptor (CD16a) (FCGR3A) antibody (FITC)

Antigen: Fc Fragment of IgG, Low Affinity IIIa, Receptor (CD16a) (FCGR3A)

Synonyms: CD16, FCG3, CD16A, FCGR3, IGFR3, FCR-10, FCRIII, FCGRIII, FCRIIIA

Clonality: Monoclonal (LNK16)

Host: Mouse

Reactivity: Human, Primates

Conjugate: FITC

Application: Flow Cytometry (FACS)

Catalog no.: ABIN93989

Additional Information

Alternative name: CD16

Immunogen: Normal human peripheral blood granulocytes

Isotype: IgG1

Clone: LNK16

Description: CD16 (FcgammaRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor.Human FcgammaRIII is expressed in two forms FcgammaRIII-A and -B. FcgammaRIII-A isa transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It isassociated with FcepsilonRI-gamma subunit and is responsible for antibody-dependent NKcell cytotoxicity. Mast cell FcgammaRIII-A is associated, moreover, with FcepsilonRI-betasubunit. Besides IgG, FcgammaRIII-A can be triggered also by oligomeric IgE.FcgammaRIII-B is a GPI-linked monomeric receptor expressed on neutrophils and isinvolved in their activation and induction of a proadhesive phenotype.

Specificity: The antibody LNK16 reacts with CD16, a low affinity receptor for aggregated IgG(FcgammaRIII antigen). CD16 exists in two different isoforms: CD16a (FcgammaRIIIA,50-65 kDa, expressed on NK-cells, monocytes and macrophages) and CD16b(FcgammaRIIIB, 48 kDa, mainly expressed on neutrophils).HLDA V, WS Code M MA069HLDA V, WS Code NK NK50

Application Details

Application Notes: The reagent is designed for Flow Cytometry analysis of human blood cells using 20 µl reagent / 100 µl of whole blood or 10 6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

Buffer: The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azideand 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.

Storage: Store in the dark at 2-8 o C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.Short-term exposure to room temperature should not affect the quality of the reagent.However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.

Research Area: Stem Cells, Hematopoietic Progenitors, Hematopoietic Stem Cells, CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only

Images

Flow Cytometry analysis

Publications

Publications: Mathison, Befus, Davison et al.: "Modulation of neutrophil function by the tripeptide feG." in: BMC immunology, Vol. 4, pp. 3, 2003 (PubMed).

Lin, Liu, Chen et al.: "CD94 1A transcripts characterize lymphoblastic lymphoma/leukemia of immature natural killer cell origin with distinct clinical features." in: Blood, Vol. 106, Issue 10, pp. 3567-74, 2005 (PubMed).

Congy-Jolivet, Bolzec, Ternant et al.: "Fc gamma RIIIa expression is not increased on natural killer cells expressing the Fc gamma RIIIa-158V allotype." in: Cancer research, Vol. 68, Issue 4, pp. 976-80, 2008 (PubMed).

Mathison, Christie, Davison: "The tripeptide feG inhibits leukocyte adhesion." in: Journal of inflammation (London, England), Vol. 5, pp. 6, 2008 (PubMed).

Chen, Freedman: "CD16+ gammadelta T cells mediate antibody dependent cellular cytotoxicity: potential mechanism in the pathogenesis of multiple sclerosis." in: Clinical immunology (Orlando, Fla.), Vol. 128, Issue 2, pp. 219-27, 2008 (PubMed).

Kahn, Moergelin, Shannon et al.: "Antibodies against a surface protein of Streptococcus pyogenes promote a pathological inflammatory response." in: PLoS pathogens, Vol. 4, Issue 9, pp. e1000149, 2008 (PubMed).

Meknache, Joensson, Laurent et al.: "Human basophils express the glycosylphosphatidylinositol-anchored low-affinity IgG receptor FcgammaRIIIB (CD16B)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 4, pp. 2542-50, 2009 (PubMed).

Mehrle, Watzl, von Lilienfeld-Toal et al.: "Comparison of phenotype of gammadelta T cells generated using various cultivation methods." in: Immunology letters, Vol. 125, Issue 1, pp. 53-8, 2009 (PubMed).