Fc Fragment of IgG, Low Affinity IIIb, Receptor (CD16b) (FCGR3B) antibody

Antigen: Fc Fragment of IgG, Low Affinity IIIb, Receptor (CD16b) (FCGR3B)

Synonyms: CD16, FCG3, CD16b, FCGR3

Clonality: Monoclonal (MEM-154)

Host: Mouse

Reactivity: Human

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB), Functional Studies (Func)

Catalog no.: ABIN93992

Additional Information

Alternative name: CD16b

Immunogen: Human granulocytes

Format: Purified

Isotype: IgG1

Clone: MEM-154

Description: CD16 (FcgammaRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor.Human FcgammaRIII is expressed in two forms FcgammaRIII-A and -B. FcgammaRIII-A isa transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It isassociated with FcepsilonRI-gamma subunit and is responsible for antibody-dependent NKcell cytotoxicity. Mast cell FcgammaRIII-A is associated, moreover, with FcepsilonRI-betasubunit. Besides IgG, FcgammaRIII-A can be triggered also by oligomeric IgE.FcgammaRIII-B is a GPI-linked monomeric receptor expressed on neutrophils and isinvolved in their activation and induction of a proadhesive phenotype.

Specificity: The antibody MEM-154 reacts with the epitope on CD16 antigen that residing in proximity toFG loop (probably BC or C'E loop). CD16 is a low affinity receptor for aggregated IgG(FcgammaRIII antigen). The antibody MEM-154 reacts with CD16+ granulocytes.HLDA V, WS Code M MA068HLDA V, WS Code NK NK51

Application Details

Application Notes: Flow Cytometry: Recommended dilution: 5-10 µg/ml. Positive control: PBL (peripheral blood lymphocytes). Application note: The antibody MEM-154 does not react with CD16a present on NK cells inmany subjects. Application note: Non-reducing conditions. Functional Application: The antibody MEM-154 blocks binding of human IgG to FcgammaRIII.

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from hybridoma culture supernatant by protein A-affinity chromatography.

Buffer: Tris buffered saline (TBS) with 15 mM sodium azide, approx. pH 8.0

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Research Area: CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only

Publications

Publications: Rose, Foley, Yi et al.: "Cholesterol is obligatory for polarization and chemotaxis but not for endocytosis and associated signaling from chemoattractant receptors in human neutrophils." in: Journal of biomedical science, Vol. 15, Issue 4, pp. 441-61, 2008 (PubMed).

Soehnlein, Kai-Larsen, Frithiof et al.: "Neutrophil primary granule proteins HBP and HNP1-3 boost bacterial phagocytosis by human and murine macrophages." in: The Journal of clinical investigation, Vol. 118, Issue 10, pp. 3491-502, 2008 (PubMed).

Jursik, Prchal, Grillari-Voglauer et al.: "Large-scale production and characterization of novel CD4+ cytotoxic T cells with broad tumor specificity for immunotherapy." in: Molecular cancer research : MCR, Vol. 7, Issue 3, pp. 339-53, 2009 (PubMed).