CD3 (CD3E) antibody

Antigen: CD3 (CD3E)

Synonyms: T3E, TCRE, FLJ18683

Clonality: Monoclonal (MEM-92)

Host: Mouse

Reactivity: Human

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Functional Studies (Func)

Catalog no.: ABIN94065

Additional Information

Alternative name: CD3

Gene ID: 11q23

Immunogen: Human peripheral blood lymphocytes.

Format: Purified

Isotype: IgM  (Matching secondary antibodies)

Clone: MEM-92

Description: CD3 complex is crucial in transducing antigen-recognition signals into the cytopasm of Tcells and in regulating the cell surface expression of the TCR complex. T-cell activationthrough the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally relatedmembers of the immunoglobulins super family encoded by closely linked genes on humanchromosome 11. The CD3 components have long cytoplasmic tails that associate withcytoplasmic signal transduction molecules. This association is mediated at least in part by adouble tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play arole in TCR-induced growth arrest, cell survival and proliferation.The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% ofthymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells.Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.

Specificity: The antibody MEM-92 reacts with epsilon chain of human CD3 complex, a part of a biggermultisubunit complex of the T cell receptor (CD3/TCR) expressed on peripheral blood Tlymphocytes and mature thymocytes.HLDA IV., WS Code T 97

Application Details

Application Notes: Functional Application: The antibody MEM-92 in solution induces early responses of T cell activation (tyrosinephosphorylation, calcium elevation, Erk activation and expression of activation antigens), butit is unable to induce T cell proliferation.

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from ascites by thiophilic adsorption-affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. Do not freeze.

Research Area: Immunology, Adaptive Immunity, Hematopoietic Progenitors, Hematopoietic Stem Cells, CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only

Publications

Publications: Stefanovua, Saville, Peters et al.: "HIV infection--induced posttranslational modification of T cell signaling molecules associated with disease progression." in: The Journal of clinical investigation, Vol. 98, Issue 6, pp. 1290-7, 1996 (PubMed).

Brdicka, Imrich, Angelisovua et al.: "Non-T cell activation linker (NTAL): a transmembrane adaptor protein involved in immunoreceptor signaling." in: The Journal of experimental medicine, Vol. 196, Issue 12, pp. 1617-26, 2002 (PubMed).

Brdicková, Brdicka, Angelisová et al.: "LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling." in: The Journal of experimental medicine, Vol. 198, Issue 10, pp. 1453-62, 2003 (PubMed).

Batista, Milluan, Mittelbrunn et al.: "Recruitment of transferrin receptor to immunological synapse in response to TCR engagement." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 172, Issue 11, pp. 6709-14, 2004 (PubMed).