CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) antibody

Antigen: CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40)

Synonyms: p50, Bp50, CDW40, MGC9013, TNFRSF5, IGM, GP39, IMD3, TRAP, HIGM1, T-BAM, Tnfrsf5, AI326936

Clonality: Monoclonal (HI40a)

Host: Mouse

Reactivity: Human

Application: Flow Cytometry (FACS)

Catalog no.: ABIN94104

Additional Information

Alternative name: CD40

Immunogen: Human CD40a

Format: Azide Free

Isotype: IgG1  (Matching secondary antibodies)

Clone: HI40a

Description: CD40 is a costimulatory molecule of the TNF receptor superfamily and is expressed onmany cell types, such as B cells, monocytes/macrophages, dendritic cells, endothelial cells,fibroblasts or vascular smooth muscle cells. Interaction of CD40 and its ligand CD154(CD40L) is required for the generation of antibody responses to T-dependent antigens aswell as for the development of germinal centers and memory B cells. Inmonocytes/macrophages CD40 engagement induces production of pro-inflammatorycytokines and chemokines. CD40-CD154 interactions are also critical for development ofCD4 T cell-dependent effector functions. CD40 links innate and adaptive immune responsesto bacterial stimuli and serves as an important regulator affecting functions of othercostimulatory molecules.

Specificity: The antibody HI40a recognizes CD40 (BP50), a 48 kDa type I single chain transmembraneglycoprotein expressed on normal and neoplastic B cells, but not on terminally differentiatedplasma cells. CD40 antigen is also present on Hodgkin's and Reed-Sternberg cells, folliculardendritic cells, some macrophages, basal epithelial cells and endothelial cells.

Application Details

Application Notes: Flow Cytometry: The purified antibody is effective for indirect immunofluorescence staining of human cells byflow cytometry analysis using 0.25 µg/test.

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from ascites by protein-G affinity chromatography.

Buffer: Azide free phosphate buffered saline (PBS), approx. pH 7.4, 0.2 µ m filter sterilized.

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Research Area: Stem Cells, Hematopoietic Progenitors, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis, Inflammation, Cancer

Restrictions: For Research Use only

Images

Flow Cytometry analysis