CD44 (CD44) antibody

Antigen: CD44 (CD44)

Synonyms: IN, LHR, MC56, MDU2, MDU3, MIC4, Pgp1, CDW44, CSPG8, HCELL, MUTCH-I, ECMR-III, MGC10468

Clonality: Monoclonal (MEM-263)

Host: Mouse

Reactivity: Human, Pig (Porcine), Dog (Canine)

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN94121

Additional Information

Alternative name: CD44

Immunogen: COS-7 cells (African Green Monkey).

Format: Purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: MEM-263

Description: CD44 is a transmembrane glycoprotein expressed on the surface of most cells, whichserves as a receptor for hyaluronan. CD44 mediates angiogenesis, cell adhesion,proliferation and migration, it is thus important for lymphocyte activation, recirculation andhoming, it can thus serve e.g. as a modulator of macrophage recruitment in response topathogen. Although CD44 functions are essential for physiological activities of normal cells,elevated CD44 expression correlates with poor prognosis in many carcinomas, facilitatingtumour growth and metastasis, antiapoptosis and directional motility of cancer cells.

Specificity: The antibody MEM-263 reacts with extracellular (N-terminal) domain of standard CD44(Phagocyte glycoprotein 1), a 80-95 kDa transmembrane glycoprotein (hyaladherin family)present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells,etc.), it is negative on platelets and hepatocytes.HLDA III, WS Code T 155

Comments:

CD44 is a transmembrane glycoprotein expressed on the surface of most cells, which serves as a receptor for hyaluronan. CD44 mediates angiogenesis, cell adhesion, proliferation and migration, it is thus important for lymphocyte activation, recirculation and homing, it can thus serve e.g. as a modulator of macrophage recruitment in response to pathogen. Although CD44 functions are essential for physiological activities of normal cells, elevated CD44 expression correlates with poor prognosis in many carcinomas, facilitating tumour growth and metastasis, antiapoptosis and directional motility of cancer cells.

Application Details

Application Notes: Flow Cytometry: Recommended dilution: 4 µg/ml. - Recommended dilution: 2 µg/ml, 60 min on vertical incubator Positive control: Kg-1a human acute leukemia cell lysateJURKAT human leukemia T-cell lysate. Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1%laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8. 2, 50 mM NaF including Proteaseinhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate withnon-reducing SDS-PAGE sample buffer. Application note: Non-reducing conditions. SDS-PAGE (6% separating gel). Immunohistochemistry (paraffin sections) - Recommended dilution: 10 µg/ml. Positive tissue: uterus, myometrium

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from ascites by protein-A affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Research Area: Stem Cells, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells, Cancer, Cell Cycle

Restrictions: For Research Use only

Images

Western Blotting analysis (non-reducing conditions) of isolated peripheral blood lymphocytes of various species using anti-CD44 (MEM-263). Lane 1: lysate of human PBL. Lane 2: lysate of canine PBL. Lane 3: lysate of porcine PBL

Publications

Publications: Subramaniam, Vincent, Gardner et al.: "CD44 regulates cell migration in human colon cancer cells via Lyn kinase and AKT phosphorylation." in: Experimental and molecular pathology, Vol. 83, Issue 2, pp. 207-15, 2007 (PubMed).

Matushansky, Hernando, Socci et al.: "A developmental model of sarcomagenesis defines a differentiation-based classification for liposarcomas." in: The American journal of pathology, Vol. 172, Issue 4, pp. 1069-80, 2008 (PubMed).

Rose, Foley, Yi et al.: "Cholesterol is obligatory for polarization and chemotaxis but not for endocytosis and associated signaling from chemoattractant receptors in human neutrophils." in: Journal of biomedical science, Vol. 15, Issue 4, pp. 441-61, 2008 (PubMed).

Klein, Weisshardt, Kleff et al.: "Vascular wall-resident CD44+ multipotent stem cells give rise to pericytes and smooth muscle cells and contribute to new vessel maturation." in: PLoS ONE, Vol. 6, Issue 5, pp. e20540, 2011 (PubMed).