CD9 (CD9) antibody

Antigen: CD9 (CD9)

Synonyms: 5H9, BA2, P24, GIG2, MIC3, MRP-1, BTCC-1, DRAP-27, TSPAN29

Clonality: Monoclonal (MEM-61)

Host: Mouse

Reactivity: Human

Application: Flow Cytometry (FACS), Western Blotting (WB), Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN94244

Additional Information

Alternative name: CD9

Gene ID: 12p13.3

Immunogen: Pre-B cell line NALM-6.

Format: Purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: MEM-61

Description: CD9 belongs to proteins of tetraspanin family that orchestrate cholesterol-associatedtetraspanin-enriched signaling microdomains within the plasma membrane, formingcomplexes with each other as well as with integrins, membrane-anchored growth factorsand other proteins. CD9 is involved in cell motility, osteoclastogenesis, neurite outgrowth,myotube formation, and sperm-egg fusion, plays roles in cell attachment and proliferationand is necessary for association of heterologous MHC II molecules on the dendritic cellplasma membrane which is important for effective T cell stimulation. CD9 is also consideredas metastasis suppressor in solid tumors.

Specificity: The antibody MEM-61 recognizes an epitope on second extracellular domain (EC2) of CD9antigen, a 24 kDa transmembrane protein expressed on platelets, monocytes, pre-Blymphocytes, granulocytes and activated T lymphocytes. HLDA VI, WS Code P P-15

Application Details

Application Notes: Western Blotting - Recommended dilution: 2-4 µg/ml. Application note: Non-reducing conditions. Immunohistochemistry (paraffin sections) - Recommended dilution: 20 µg/ml. Positive tissue: prostate. Functional Application: The antibody MEM-61 induces Fc γ R-dependent platelet aggregation.

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from ascites by protein-A affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Research Area: Embryogenesis, Embryonic stem cells, Reproduction, CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only

Publications

Publications: Stöckl, Majdic, Fischer et al.: "Monomorphic molecules function as additional recognition structures on haptenated target cells for HLA-A1-restricted, hapten-specific CTL." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 5, pp. 2724-33, 2001 (PubMed).

Singh, Sugimoto, Dhawan et al.: "Juxtacrine activation of EGFR regulates claudin expression and increases transepithelial resistance." in: American journal of physiology. Cell physiology, Vol. 293, Issue 5, pp. C1660-8, 2007 (PubMed).

Lafleur, Xu, Hemler: "Tetraspanin proteins regulate membrane type-1 matrix metalloproteinase-dependent pericellular proteolysis." in: Molecular biology of the cell, Vol. 20, Issue 7, pp. 2030-40, 2009 (PubMed).