C Peptide of Proinsulin antibody

Antigen: C Peptide of Proinsulin

Clonality: Monoclonal (C-PEP-01)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Radioimmunoassay (RIA), Immunocytochemistry (ICC)

Catalog no.: ABIN94267

Additional Information

Alternative name: C-peptide of Proinsulin

Immunogen: Human C-peptide conjugated to bovine serum albumin.

Format: Purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: C-PEP-01

Description: From every molecule of Proinsulin, one molecule of Insulin plus one molecule of C-peptideare produced. C-peptide is released into the blood stream in equal amounts to Insulin.

Specificity: The antibody C-PEP-01 reacts specifically with C-peptide, a part of the Proinsulin molecule.Proinsulin consists of the three parts: C-peptide and two long strands of amino acids (alphaand beta chains, later become linked together to form the Insulin molecule).No cross-reactivity with Insulin or other peptide hormones or proteins was observed.

Application Details

Application Notes: Immunohistochemistry (paraffin sections) - Recommended dilution: purified 2-5 µg/ml biotin 25 µg/ml. Positive control: human pancreas (islets of Langerhans)

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from ascites by precipitation methods.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Restrictions: For Research Use only

Images

Immunohistochemistry of paraffin-embedded sections (human pancreas)

Publications

Publications: Koblas, Zacharovova, Berkova et al.: "An acidic pH and activation of phosphoinositide 3-kinase stimulate differentiation of pancreatic progenitors into insulin-producing cells." in: Transplantation proceedings, Vol. 42, Issue 6, pp. 2075-80, 2010 (PubMed).