Keratin (KRT) (pan) antibody

Antigen: Keratin (KRT)

Synonyms: K1, CK1, EHK, EHK1, EPPK, KRT1A, NEPPK

Epitope: pan

Clonality: Monoclonal (C-11)

Host: Mouse

Reactivity: Mammalian

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Catalog no.: ABIN94275

Additional Information

Alternative name: Cytokeratin (Pan-reactive)

Immunogen: Keratin-enriched preparation from human epidermoid carcinoma cell line A431.

Format: Purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: C-11

Description: Cytokeratins are a subfamily of intermediate filaments and characterized by remarkablebiochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20different polypeptides, molecular weight between 40 kDa and 68 kDa. The individualcytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidiccytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.

Specificity: The antibody C-11 reacts with Cytokeratin peptides 4, 5, 6, 8, 10, 13, 18. Cytokeratins are amember of intermediate filaments subfamily represented in epithelial tissues.

Application Details

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from ascites by protein-A affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Restrictions: For Research Use only

Images

Detection of cytokeratin on paraffin-embedded sections of guinea pig breast carcinoma using anti-cytokeratin antibody (C-11).

Intracellular Flow Cytometry analysis

Publications

Publications: Bártek, Vojtĕsek, Stasková et al.: "A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology." in: The Journal of pathology, Vol. 164, Issue 3, pp. 215-24, 1991 (PubMed).

Kovaruik, Rejthar, Lauerovua et al.: "Monoclonal antibodies against individual cytokeratins in the detection of metastatic spread." in: International journal of cancer. Supplement = Journal international du cancer. Supplement, Vol. 3, pp. 50-5, 1989 (PubMed).

Hamakawa, Sumida, Tanioka et al.: "Extraction of cytokeratin from the human submandibular gland and its electrophoretic analysis." in: Research communications in molecular pathology and pharmacology, Vol. 101, Issue 2, pp. 115-26, 1999 (PubMed).

Broekema, Harmsen, Koerts et al.: "Determinants of tubular bone marrow-derived cell engraftment after renal ischemia/reperfusion in rats." in: Kidney international, Vol. 68, Issue 6, pp. 2572-81, 2005 (PubMed).

Bianchi, Bombelli, Raimondo et al.: "Primary cell cultures from human renal cortex and renal-cell carcinoma evidence a differential expression of two spliced isoforms of Annexin A3." in: The American journal of pathology, Vol. 176, Issue 4, pp. 1660-70, 2010 (PubMed).

Haun, Devaraj, Marinelli et al.: "Probing intracellular biomarkers and mediators of cell activation using nanosensors and bioorthogonal chemistry." in: ACS nano, Vol. 5, Issue 4, pp. 3204-13, 2011 (PubMed).